Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB4851-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Hamster Monoclonal IFN-gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma in direct ELISAs. Does not cross-react with rat, cotton rat, human, rhesus macaque, porcine, feline, or canine IFN-gamma .
- Reactivity
- Mouse
- Host
- Hamster
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB4851). The ND50 is typically 4-20 ng/mL.