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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB5851 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IFN-gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat IFN-gamma in ELISAs. In sandwich ELISAs, observed cross-reactivities were less than 2% with recombinant mouse IFN-gamma and less than 0.1% with recombinant human IFN-gamma and recombinant porcine IFN-gamma .
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01581- Isotype
- IgG
- Antibody clone number
- 88928
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
Stem cells (Dayton, Ohio) 2008 Apr;26(4):1047-55
Stem cells (Dayton, Ohio) 2008 Apr;26(4):1047-55
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Roche M, Diamond M, Kelly JP, Finn DP
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma in Rat Splenocytes. IFN-gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Mouse Anti-Rat IFN-gamma Monoclonal Antibody (Catalog # MAB5851) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
