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- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB485-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IFN-gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN-gamma , rrIFN-gamma , rpIFN-gamma , rrmIFN-gamma , rfeIFN-gamma , or rcaIFN-gamma .
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 37895
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis.
Crucial role of OX40/OX40L signaling in a murine model of asthma.
CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction.
Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression.
IL-21 promotes skin recruitment of CD4(+) cells and drives IFN-γ-dependent epidermal hyperplasia.
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase.
Maternal immune activation alters fetal brain development through interleukin-6.
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Nam Y, Jung SM, Rim YA, Jung H, Lee K, Park N, Kim J, Jang Y, Park YB, Park SH, Ju JH
PloS one 2018;13(6):e0198740
PloS one 2018;13(6):e0198740
Crucial role of OX40/OX40L signaling in a murine model of asthma.
Lei W, Zeng D, Liu G, Zhu Y, Wang J, Wu H, Jiang J, Huang J
Molecular medicine reports 2018 Mar;17(3):4213-4220
Molecular medicine reports 2018 Mar;17(3):4213-4220
CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction.
He W, Yang C, Xia L, Zhao MZ, Ge RT, Huang H, Ji Q, Yang PC, Liu Z
Cytokine 2014 Jul;68(1):32-9
Cytokine 2014 Jul;68(1):32-9
Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression.
Yoo HS, Choi Y, Ahn N, Lee S, Kim WU, Jang MS, Jang MH, Kim YS, Yoo JY
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1440-8
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1440-8
IL-21 promotes skin recruitment of CD4(+) cells and drives IFN-γ-dependent epidermal hyperplasia.
Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2011 May 1;186(9):5435-42
Journal of immunology (Baltimore, Md. : 1950) 2011 May 1;186(9):5435-42
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
Jin W, Qi S, Luo H
BMC immunology 2011 Dec 19;12:68
BMC immunology 2011 Dec 19;12:68
IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
Deepak P, Kumar S Jr, Kishore D, Acharya A
International immunology 2010 Jan;22(1):53-63
International immunology 2010 Jan;22(1):53-63
Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 15;184(8):4159-69
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 15;184(8):4159-69
TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4089-97
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4089-97
Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase.
Iwanami K, Matsumoto I, Tanaka-Watanabe Y, Inoue A, Mihara M, Ohsugi Y, Mamura M, Goto D, Ito S, Tsutsumi A, Kishimoto T, Sumida T
Arthritis and rheumatism 2008 Mar;58(3):754-63
Arthritis and rheumatism 2008 Mar;58(3):754-63
Maternal immune activation alters fetal brain development through interleukin-6.
Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 3;27(40):10695-702
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 3;27(40):10695-702
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
Journal of molecular and cellular cardiology 2006 Nov;41(5):885-92
Journal of molecular and cellular cardiology 2006 Nov;41(5):885-92
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IFN-gamma in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485). The ND50 is typically 0.075-0.3 µg/mL.
