Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC285P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-gamma PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma , recombinant rat IFN-gamma , or recombinant porcine IFN-gamma is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
AAP20098.1
- Isotype
- IgG
- Antibody clone number
- 25723
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease.
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases.
Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Identification of human NK17/NK1 cells.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, Kong F
Cytokine 2016 Sep;85:37-44
Cytokine 2016 Sep;85:37-44
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova JL, Puel A
The Journal of experimental medicine 2015 May 4;212(5):619-31
The Journal of experimental medicine 2015 May 4;212(5):619-31
Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins JP, Reagan K, Kalyuzhny AE
Cells 2015 Feb 11;4(1):84-95
Cells 2015 Feb 11;4(1):84-95
High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic SS, Caragol I, Baumann U, Klein C, Welte K, Hansen G
The Journal of allergy and clinical immunology 2014 Jan;133(1):172-80.e1-10
The Journal of allergy and clinical immunology 2014 Jan;133(1):172-80.e1-10
Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases.
Chang CX, Tan AT, Or MY, Toh KY, Lim PY, Chia AS, Froesig TM, Nadua KD, Oh HL, Leong HN, Hadrup SR, Gehring AJ, Tan YJ, Bertoletti A, Grotenbreg GM
European journal of immunology 2013 Apr;43(4):1109-20
European journal of immunology 2013 Apr;43(4):1109-20
Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
Silk KM, Silk JD, Ichiryu N, Davies TJ, Nolan KF, Leishman AJ, Carpenter L, Watt SM, Cerundolo V, Fairchild PJ
Gene therapy 2012 Oct;19(10):1035-40
Gene therapy 2012 Oct;19(10):1035-40
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Glässner A, Eisenhardt M, Krämer B, Körner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Identification of human NK17/NK1 cells.
Pandya AD, Al-Jaderi Z, Høglund RA, Holmøy T, Harbo HF, Norgauer J, Maghazachi AA
PloS one 2011;6(10):e26780
PloS one 2011;6(10):e26780
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
Clinical and experimental immunology 2006 Sep;145(3):438-47
Clinical and experimental immunology 2006 Sep;145(3):438-47
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
The American journal of pathology 2004 Feb;164(2):409-17
The American journal of pathology 2004 Feb;164(2):409-17
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IFN-gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC285P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.