Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC285F-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-gamma Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma , recombinant rat IFN-gamma , or recombinant porcine IFN-gamma is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
AAP20098.1
- Isotype
- IgG
- Antibody clone number
- 25723
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Trichinella spiralis Excretory-Secretory Products Induce Tolerogenic Properties in Human Dendritic Cells via Toll-Like Receptors 2 and 4.
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity.
Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
IL-10-producing regulatory B cells in the pathogenesis of chronic hepatitis B virus infection.
NF-kappa B modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gamma delta lymphocytes.
Ilic N, Gruden-Movsesijan A, Cvetkovic J, Tomic S, Vucevic DB, Aranzamendi C, Colic M, Pinelli E, Sofronic-Milosavljevic L
Frontiers in immunology 2018;9:11
Frontiers in immunology 2018;9:11
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X
Scientific reports 2016 Sep 2;6:32320
Scientific reports 2016 Sep 2;6:32320
B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity.
Piancone F, Saresella M, Marventano I, La Rosa F, Zoppis M, Agostini S, Longhi R, Caputo D, Mendozzi L, Rovaris M, Clerici M
Scientific reports 2016 Jul 14;6:29699
Scientific reports 2016 Jul 14;6:29699
Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
Chen Y, Ayaru L, Mathew S, Morris E, Pereira SP, Behboudi S
PloS one 2014;9(2):e88133
PloS one 2014;9(2):e88133
IL-10-producing regulatory B cells in the pathogenesis of chronic hepatitis B virus infection.
Das A, Ellis G, Pallant C, Lopes AR, Khanna P, Peppa D, Chen A, Blair P, Dusheiko G, Gill U, Kennedy PT, Brunetto M, Lampertico P, Mauri C, Maini MK
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 15;189(8):3925-35
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 15;189(8):3925-35
NF-kappa B modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gamma delta lymphocytes.
Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):5857-64
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):5857-64
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IFN-gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 μg/mL Ionomycin, and 3 μM Monensin overnight were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN-gamma Fluorescein-conjugated Monoclonal Antibody (Catalog # IC285F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.