Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF781 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant porcine IFN-gamma is observed and less than 5% cross-reactivity with recombinant human IFN-gamma , recombinant rhesus macaque IFN-gamma , recombinant mouse IFN-gamma , and recombinant rat IFN-gamma is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P42161
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mechanisms of Immune Suppression Utilized by Canine Adipose and Bone Marrow-Derived Mesenchymal Stem Cells.
Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice.
Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccine to protect cats.
Chow L, Johnson V, Coy J, Regan D, Dow S
Stem cells and development 2017 Mar 1;26(5):374-389
Stem cells and development 2017 Mar 1;26(5):374-389
Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice.
Kim CG, Lee JE, Jeong DG, Lee YH, Park SI, Lee DG, Han CH, Kang SJ, Song CH, Choi SH, Lee YJ, Ku SK
Experimental and therapeutic medicine 2017 Jun;13(6):3448-3466
Experimental and therapeutic medicine 2017 Jun;13(6):3448-3466
Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccine to protect cats.
Cupillard L, Juillard V, Latour S, Colombet G, Cachet N, Richard S, Blanchard S, Fischer L
Vaccine 2005 Mar 14;23(16):1910-6
Vaccine 2005 Mar 14;23(16):1910-6
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma in Canine PBMCs. IFN-gamma was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) untreated (lower panel) or treated with PMA and calcium ionomycin (upper panel) using Goat Anti-Canine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of VSV-induced Cytopathy and Neutralization by Canine IFN-gamma Antibody. Recombinant Canine IFN-gamma (Catalog # 781-CG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the A-72 canine fibroma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Canine IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781). The ND50 is typically 0.2-1 µg/mL.