Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- BAF781 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine IFN-gamma Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine IFN-gamma in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IFN-gamma is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P42161
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial.
Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs.
Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
Lemesre JL, Holzmuller P, Gonçalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G
Vaccine 2007 May 22;25(21):4223-34
Vaccine 2007 May 22;25(21):4223-34
Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
Vaccine 2006 Jan 23;24(4):501-9
Vaccine 2006 Jan 23;24(4):501-9
Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs.
Strauss-Ayali D, Baneth G, Shor S, Okano F, Jaffe CL
International journal for parasitology 2005 Jan;35(1):63-73
International journal for parasitology 2005 Jan;35(1):63-73
Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
Lemesre JL, Holzmuller P, Cavaleyra M, Gonçalves RB, Hottin G, Papierok G
Vaccine 2005 Apr 22;23(22):2825-40
Vaccine 2005 Apr 22;23(22):2825-40
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- IGFBP-1 in Canine PBMCs. IGFBP-1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Human IGFBP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF871) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.