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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF2300 - Provider product page
- Provider
- R&D Systems
- Product name
- Bovine IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects bovine IFN-gamma in direct ELISAs and Western blots.
- Reactivity
- Bovine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_776511- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma in Bovine PBMCs. IFN-gamma was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Bovine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
