Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF985 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant canine IFN-gamma and recombinant feline IFN-gamma is observed, 10% cross-reactivity with recombinant equine IFN-gamma and recombinant human IFN-gamma is observed, and less than 5% cross-reactivity with recombinant mouse IFN-gamma , recombinant rat IFN-gamma , recombinant cotton rat IFN-gamma , and recombinant rhesus macaque IFN-gamma is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P17803
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Porcine IFN-gamma Antibody. Recombinant Porcine IFN-gamma (Catalog # 985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985). The ND50 is typically 0.6-3.0 µg/mL.