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- Antibody Data
- Antigen structure
- References [84]
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- Western blot [3]
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- Product number
- MM700B - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFN gamma Monoclonal Antibody (XMG1.2), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MM700B targets IFN gamma in ELISA, IHC-F, Neutralization and WB applications and shows reactivity with mouse and human samples. The MM700B immunogen is recombinant mouse IFN gamma. MM700B detects IFN gamma which has a predicted molecular weight of approximately 16 kDa. The MM700B mouse GMCSF antibody (biotinylated conjugate of clone XMG1.2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM700 (same clone). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody MM700B (clone XMG1.2) and antibody MM700 (same clone) have successfully been used in combination with recombinant mouse IFNg protein SMIFNg in ELISA applications. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- XMG1.2
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Up-Regulated Expression of Interferon-Gamma, Interleukin-6 and Tumor Necrosis Factor-Alpha in the Endolymphatic Sac of Meniere's Disease Suggesting the Local Inflammatory Response Underlies the Mechanism of This Disease.
Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
An atypical CD8 T-cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin-13.
Anti-interleukin-10R1 monoclonal antibody in combination with bacillus Calmette--Guérin is protective against bladder cancer metastasis in a murine orthotopic tumour model and demonstrates systemic specific anti-tumour immunity.
Perforin is detrimental to controlling [corrected] C. muridarum replication in vitro, but not in vivo.
Imiquimod attenuates the growth of UVB-induced SCC in mice through Th1/Th17 cells.
The secreted lipoprotein, MPT83, of Mycobacterium tuberculosis is recognized during human tuberculosis and stimulates protective immunity in mice.
Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo.
PmpG303-311, a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice.
Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract.
Urothelial antigen-specific CD4+ T cells function as direct effector cells and induce bladder autoimmune inflammation independent of CD8+ T cells.
Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes.
Sensitization with anti-inflammatory BmAFI of Brugia malayi allows L3 development in the hostile peritoneal cavity of Mastomys coucha.
Peroxisome proliferator-activated receptor delta agonists inhibit T helper type 1 (Th1) and Th17 responses in experimental allergic encephalomyelitis.
Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes.
Theiler's virus infection induces a predominant pathogenic CD4+ T cell response to RNA polymerase in susceptible SJL/J mice.
Immunological diversity within a family of cutinase-like proteins of Mycobacterium tuberculosis.
Evasion of peptide, but not lipid antigen presentation, through pathogen-induced dendritic cell maturation.
Phage display of a CTL epitope elicits a long-term in vivo cytotoxic response.
Oral attenuated Salmonella enterica serovar Typhimurium vaccine expressing codon-optimized HIV type 1 Gag enhanced intestinal immunity in mice.
Cytolysin-dependent escape of the bacterium from the phagosome is required but not sufficient for induction of the Th1 immune response against Listeria monocytogenes infection: distinct role of Listeriolysin O determined by cytolysin gene replacement.
Gamma interferon, tumor necrosis factor alpha, and nitric oxide synthase 2, key elements of cellular immunity, perform critical protective functions during humoral defense against lethal pulmonary Yersinia pestis infection.
IL-2 induces expression and secretion of IFN-gamma in murine peritoneal macrophages.
Listeriolysin O, a cytolysin derived from Listeria monocytogenes, inhibits generation of ovalbumin-specific Th2 immune response by skewing maturation of antigen-specific T cells into Th1 cells.
Independent protective effects for tumor necrosis factor and lymphotoxin alpha in the host response to Listeria monocytogenes infection.
Trypanosoma cruzi: the expansion of NK, T, and NKT cells in the experimental infection.
T cell-derived tumour necrosis factor is essential, but not sufficient, for protection against Mycobacterium tuberculosis infection.
Studies on the generation and maintenance of mucosal cytotoxic T lymphocytes against human immunodeficiency virus type 1 Gag in mice.
The combination of plasmid interleukin-12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette-Guèrin) in protecting against systemic Mycobacterim avium infection.
Induction of mucosal tolerance with recombinant Hev b 1 and recombinant Hev b 3 for prevention of latex allergy in BALB/c mice.
Regulation of NK cell function in vivo by the dose of tumour transplanted in the peritoneum.
Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.
Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+))CD11b(+ )immature myeloid suppressor cells.
TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection.
TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection.
Dissociated linkage of cytokine-inducing activity and cytotoxicity to different domains of listeriolysin O from Listeria monocytogenes.
Essential role of interleukin-12 (IL-12) and IL-18 for gamma interferon production induced by listeriolysin O in mouse spleen cells.
Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production.
The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.
Cancer immunotherapy with interleukin 12 and granulocyte-macrophage colony-stimulating factor-encapsulated microspheres: coinduction of innate and adaptive antitumor immunity and cure of disseminated disease.
Coexpression of interleukin-12 chains by a self-splicing vector increases the protective cellular immune response of DNA and Mycobacterium bovis BCG vaccines against Mycobacterium tuberculosis.
Effect of targeted disruption of STAT4 and STAT6 on the induction of experimental autoimmune encephalomyelitis.
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
CD28 costimulation is required not only to induce IL-12 receptor but also to render janus kinases/STAT4 responsive to IL-12 stimulation in TCR-triggered T cells.
CD28-independent induction of experimental autoimmune encephalomyelitis.
Positive regulatory role of IL-12 in macrophages and modulation by IFN-gamma.
Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice.
DNA encoding a single mycobacterial antigen protects against leprosy infection.
Dietary ribonucleotides modulate type 1 and type 2 T-helper cell responses against ovalbumin in young BALB/cJ mice.
A light, nontoxic interleukin 12 protocol inhibits HER-2/neu mammary carcinogenesis in BALB/c transgenic mice with established hyperplasia.
Partially TAP-independent protection against Listeria monocytogenes by H2-M3-restricted CD8+ T cells.
Tumor vaccination that enhances antitumor T-cell responses does not inhibit the growth of established tumors even in combination with interleukin-12 treatment: the importance of inducing intratumoral T-cell migration.
Mycobacterium bovis bacillus calmette-guérin induces protective immunity against infection by Plasmodium yoelii at blood-stage depending on shifting immunity toward Th1 type and inducing protective IgG2a after the parasite infection.
Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models.
Protection against virulent Mycobacterium avium infection following DNA vaccination with the 35-kilodalton antigen is accompanied by induction of gamma interferon-secreting CD4(+) T cells.
Nitric oxide-producing CD11b(+)Ly-6G(Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice.
Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis.
Cytokine requirements for production of a novel anti-CD8-resistant CTL population.
The absence of interleukin 1 receptor-related T1/ST2 does not affect T helper cell type 2 development and its effector function.
Structural deficiencies in granuloma formation in TNF gene-targeted mice underlie the heightened susceptibility to aerosol Mycobacterium tuberculosis infection, which is not compensated for by lymphotoxin.
A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system.
Mouse monocyte-derived chemokine is involved in airway hyperreactivity and lung inflammation.
Co-immunization with DNA vaccines expressing granulocyte-macrophage colony-stimulating factor and mycobacterial secreted proteins enhances T-cell immunity, but not protective efficacy against Mycobacterium tuberculosis.
Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis.
IL-4-independent induction of airway hyperresponsiveness by Th2, but not Th1, cells.
Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.
A critical role for IL-18 in the proliferation and activation of NK1.1+ CD3- cells.
Sequential T cell response involved in tumor rejection of sarcoma, Meth A, in syngeneic mice.
Endogenous myelin basic protein inactivates the high avidity T cell repertoire.
Topical FK506 suppresses cytokine and costimulatory molecule expression in epidermal and local draining lymph node cells during primary skin immune responses.
Perforin and IFN-gamma are involved in the antitumor effects of antibody-targeted superantigens.
Cardiac allografts from IL-4 knockout recipients: assessment of transplant arteriosclerosis and peripheral tolerance.
Requirement for distinct Janus kinases and STAT proteins in T cell proliferation versus IFN-gamma production following IL-12 stimulation.
B cell-mediated down-regulation of IFN-gamma and IL-12 production induced during anti-tumor immune responses in the tumor-bearing state.
Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18.
A mechanism underlying synergy between IL-12 and IFN-gamma-inducing factor in enhanced production of IFN-gamma.
Humoral and cellular immunity in secondary infection due to murine Chlamydia trachomatis.
The cytokines IL-4, IFN-gamma, and IL-12 regulate the development of subsets of memory effector helper T cells in vitro.
A role for nonspecific (cyclosporin A) or specific (monoclonal antibodies to ICAM-1, LFA-1, and IL-10) immunomodulation in the prolongation of skin allografts after antigen-specific pretransplant immunization or transfusion.
Effects of IL-12 on in vivo cytokine gene expression and Ig isotype selection.
Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.
Transfected Leishmania expressing biologically active IFN-gamma.
IFN-gamma involvement in the severity of gram-negative infections in mice.
Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5.
Huang C, Wang Q, Pan X, Li W, Liu W, Jiang W, Huang L, Peng A, Zhang Z
Frontiers in neurology 2022;13:781031
Frontiers in neurology 2022;13:781031
Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
Chang ZY, Liu HM, Leu YL, Hsu CH, Lee TY
International journal of molecular sciences 2022 Feb 28;23(5)
International journal of molecular sciences 2022 Feb 28;23(5)
An atypical CD8 T-cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin-13.
Johnson RM, Kerr MS, Slaven JE
Immunology 2014 Jun;142(2):248-57
Immunology 2014 Jun;142(2):248-57
Anti-interleukin-10R1 monoclonal antibody in combination with bacillus Calmette--Guérin is protective against bladder cancer metastasis in a murine orthotopic tumour model and demonstrates systemic specific anti-tumour immunity.
Newton MR, Askeland EJ, Andresen ED, Chehval VA, Wang X, Askeland RW, O'Donnell MA, Luo Y
Clinical and experimental immunology 2014 Jul;177(1):261-8
Clinical and experimental immunology 2014 Jul;177(1):261-8
Perforin is detrimental to controlling [corrected] C. muridarum replication in vitro, but not in vivo.
Johnson RM, Kerr MS, Slaven JE
PloS one 2013;8(5):e63340
PloS one 2013;8(5):e63340
Imiquimod attenuates the growth of UVB-induced SCC in mice through Th1/Th17 cells.
Yokogawa M, Takaishi M, Nakajima K, Kamijima R, Digiovanni J, Sano S
Molecular carcinogenesis 2013 Oct;52(10):760-9
Molecular carcinogenesis 2013 Oct;52(10):760-9
The secreted lipoprotein, MPT83, of Mycobacterium tuberculosis is recognized during human tuberculosis and stimulates protective immunity in mice.
Kao FF, Mahmuda S, Pinto R, Triccas JA, West NP, Britton WJ
PloS one 2012;7(5):e34991
PloS one 2012;7(5):e34991
Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo.
Kumalasari ID, Harmayani E, Lestari LA, Raharjo S, Asmara W, Nishi K, Sugahara T
Cytotechnology 2012 Mar;64(2):131-7
Cytotechnology 2012 Mar;64(2):131-7
PmpG303-311, a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice.
Johnson RM, Yu H, Kerr MS, Slaven JE, Karunakaran KP, Brunham RC
Infection and immunity 2012 Jun;80(6):2204-11
Infection and immunity 2012 Jun;80(6):2204-11
Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract.
Johnson RM, Kerr MS, Slaven JE
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):1896-904
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):1896-904
Urothelial antigen-specific CD4+ T cells function as direct effector cells and induce bladder autoimmune inflammation independent of CD8+ T cells.
Liu W, Chen X, Evanoff DP, Luo Y
Mucosal immunology 2011 Jul;4(4):428-37
Mucosal immunology 2011 Jul;4(4):428-37
Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes.
Erikçi E, Gursel M, Gürsel I
Biomaterials 2011 Feb;32(6):1715-23
Biomaterials 2011 Feb;32(6):1715-23
Sensitization with anti-inflammatory BmAFI of Brugia malayi allows L3 development in the hostile peritoneal cavity of Mastomys coucha.
Joseph SK, Verma SK, Sahoo MK, Dixit S, Verma AK, Kushwaha V, Saxena K, Sharma A, Saxena JK, Murthy PK
Acta tropica 2011 Dec;120(3):191-205
Acta tropica 2011 Dec;120(3):191-205
Peroxisome proliferator-activated receptor delta agonists inhibit T helper type 1 (Th1) and Th17 responses in experimental allergic encephalomyelitis.
Kanakasabai S, Chearwae W, Walline CC, Iams W, Adams SM, Bright JJ
Immunology 2010 Aug;130(4):572-88
Immunology 2010 Aug;130(4):572-88
Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes.
Hanafi LA, Bolduc M, Gagné ME, Dufour F, Langelier Y, Boulassel MR, Routy JP, Leclerc D, Lapointe R
Vaccine 2010 Aug 2;28(34):5617-26
Vaccine 2010 Aug 2;28(34):5617-26
Theiler's virus infection induces a predominant pathogenic CD4+ T cell response to RNA polymerase in susceptible SJL/J mice.
Jin YH, Kang B, Kim BS
Journal of virology 2009 Nov;83(21):10981-92
Journal of virology 2009 Nov;83(21):10981-92
Immunological diversity within a family of cutinase-like proteins of Mycobacterium tuberculosis.
West NP, Wozniak TM, Valenzuela J, Feng CG, Sher A, Ribeiro JM, Britton WJ
Vaccine 2008 Jul 23;26(31):3853-9
Vaccine 2008 Jul 23;26(31):3853-9
Evasion of peptide, but not lipid antigen presentation, through pathogen-induced dendritic cell maturation.
Hava DL, van der Wel N, Cohen N, Dascher CC, Houben D, León L, Agarwal S, Sugita M, van Zon M, Kent SC, Shams H, Peters PJ, Brenner MB
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 12;105(32):11281-6
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 12;105(32):11281-6
Phage display of a CTL epitope elicits a long-term in vivo cytotoxic response.
Mascolo D, Barba P, De Berardinis P, Di Rosa F, Del Pozzo G
FEMS immunology and medical microbiology 2007 Jun;50(1):59-66
FEMS immunology and medical microbiology 2007 Jun;50(1):59-66
Oral attenuated Salmonella enterica serovar Typhimurium vaccine expressing codon-optimized HIV type 1 Gag enhanced intestinal immunity in mice.
Tsunetsugu-Yokota Y, Ishige M, Murakami M
AIDS research and human retroviruses 2007 Feb;23(2):278-86
AIDS research and human retroviruses 2007 Feb;23(2):278-86
Cytolysin-dependent escape of the bacterium from the phagosome is required but not sufficient for induction of the Th1 immune response against Listeria monocytogenes infection: distinct role of Listeriolysin O determined by cytolysin gene replacement.
Hara H, Kawamura I, Nomura T, Tominaga T, Tsuchiya K, Mitsuyama M
Infection and immunity 2007 Aug;75(8):3791-801
Infection and immunity 2007 Aug;75(8):3791-801
Gamma interferon, tumor necrosis factor alpha, and nitric oxide synthase 2, key elements of cellular immunity, perform critical protective functions during humoral defense against lethal pulmonary Yersinia pestis infection.
Parent MA, Wilhelm LB, Kummer LW, Szaba FM, Mullarky IK, Smiley ST
Infection and immunity 2006 Jun;74(6):3381-6
Infection and immunity 2006 Jun;74(6):3381-6
IL-2 induces expression and secretion of IFN-gamma in murine peritoneal macrophages.
Puddu P, Carollo M, Pietraforte I, Spadaro F, Tombesi M, Ramoni C, Belardelli F, Gessani S
Journal of leukocyte biology 2005 Sep;78(3):686-95
Journal of leukocyte biology 2005 Sep;78(3):686-95
Listeriolysin O, a cytolysin derived from Listeria monocytogenes, inhibits generation of ovalbumin-specific Th2 immune response by skewing maturation of antigen-specific T cells into Th1 cells.
Yamamoto K, Kawamura I, Tominaga T, Nomura T, Kohda C, Ito J, Mitsuyama M
Clinical and experimental immunology 2005 Nov;142(2):268-74
Clinical and experimental immunology 2005 Nov;142(2):268-74
Independent protective effects for tumor necrosis factor and lymphotoxin alpha in the host response to Listeria monocytogenes infection.
Roach DR, Briscoe H, Saunders BM, Britton WJ
Infection and immunity 2005 Aug;73(8):4787-92
Infection and immunity 2005 Aug;73(8):4787-92
Trypanosoma cruzi: the expansion of NK, T, and NKT cells in the experimental infection.
Antúnez MI, Cardoni RL
Experimental parasitology 2004 Mar-Apr;106(3-4):85-94
Experimental parasitology 2004 Mar-Apr;106(3-4):85-94
T cell-derived tumour necrosis factor is essential, but not sufficient, for protection against Mycobacterium tuberculosis infection.
Saunders BM, Briscoe H, Britton WJ
Clinical and experimental immunology 2004 Aug;137(2):279-87
Clinical and experimental immunology 2004 Aug;137(2):279-87
Studies on the generation and maintenance of mucosal cytotoxic T lymphocytes against human immunodeficiency virus type 1 Gag in mice.
Yoshizawa I, Mizuochi T, Ogata A, Murakami M, Yagita H, Takahashi Y, Mizuochi T, Takemori T, Tsunetsugu-Yokota Y
AIDS research and human retroviruses 2003 Jun;19(6):469-79
AIDS research and human retroviruses 2003 Jun;19(6):469-79
The combination of plasmid interleukin-12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette-Guèrin) in protecting against systemic Mycobacterim avium infection.
Martin E, Kamath AT, Briscoe H, Britton WJ
Immunology 2003 Jun;109(2):308-14
Immunology 2003 Jun;109(2):308-14
Induction of mucosal tolerance with recombinant Hev b 1 and recombinant Hev b 3 for prevention of latex allergy in BALB/c mice.
Hufnagl K, Wagner B, Winkler B, Baier K, Hochreiter R, Thalhamer J, Kraft D, Scheiner O, Breiteneder H, Wiedermann U
Clinical and experimental immunology 2003 Aug;133(2):170-6
Clinical and experimental immunology 2003 Aug;133(2):170-6
Regulation of NK cell function in vivo by the dose of tumour transplanted in the peritoneum.
Das S, Khar A
Immunology letters 2002 Sep 2;83(2):133-42
Immunology letters 2002 Sep 2;83(2):133-42
Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.
Chen M, Hu KF, Rozell B, Orvell C, Morein B, Liljeström P
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3208-16
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3208-16
Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+))CD11b(+ )immature myeloid suppressor cells.
Goñi O, Alcaide P, Fresno M
International immunology 2002 Oct;14(10):1125-34
International immunology 2002 Oct;14(10):1125-34
TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection.
Roach DR, Bean AG, Demangel C, France MP, Briscoe H, Britton WJ
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4620-7
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4620-7
TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection.
Roach DR, Bean AG, Demangel C, France MP, Briscoe H, Britton WJ
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4620-7
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4620-7
Dissociated linkage of cytokine-inducing activity and cytotoxicity to different domains of listeriolysin O from Listeria monocytogenes.
Kohda C, Kawamura I, Baba H, Nomura T, Ito Y, Kimoto T, Watanabe I, Mitsuyama M
Infection and immunity 2002 Mar;70(3):1334-41
Infection and immunity 2002 Mar;70(3):1334-41
Essential role of interleukin-12 (IL-12) and IL-18 for gamma interferon production induced by listeriolysin O in mouse spleen cells.
Nomura T, Kawamura I, Tsuchiya K, Kohda C, Baba H, Ito Y, Kimoto T, Watanabe I, Mitsuyama M
Infection and immunity 2002 Mar;70(3):1049-55
Infection and immunity 2002 Mar;70(3):1049-55
Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production.
Angulo I, Jiménez-Díaz MB, García-Bustos JF, Gargallo D, de las Heras FG, Muñoz-Fernández MA, Fresno M
Cellular immunology 2002 Jul-Aug;218(1-2):46-58
Cellular immunology 2002 Jul-Aug;218(1-2):46-58
The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.
Smith RS, Harris SG, Phipps R, Iglewski B
Journal of bacteriology 2002 Feb;184(4):1132-9
Journal of bacteriology 2002 Feb;184(4):1132-9
Cancer immunotherapy with interleukin 12 and granulocyte-macrophage colony-stimulating factor-encapsulated microspheres: coinduction of innate and adaptive antitumor immunity and cure of disseminated disease.
Hill HC, Conway TF Jr, Sabel MS, Jong YS, Mathiowitz E, Bankert RB, Egilmez NK
Cancer research 2002 Dec 15;62(24):7254-63
Cancer research 2002 Dec 15;62(24):7254-63
Coexpression of interleukin-12 chains by a self-splicing vector increases the protective cellular immune response of DNA and Mycobacterium bovis BCG vaccines against Mycobacterium tuberculosis.
Palendira U, Kamath AT, Feng CG, Martin E, Chaplin PJ, Triccas JA, Britton WJ
Infection and immunity 2002 Apr;70(4):1949-56
Infection and immunity 2002 Apr;70(4):1949-56
Effect of targeted disruption of STAT4 and STAT6 on the induction of experimental autoimmune encephalomyelitis.
Chitnis T, Najafian N, Benou C, Salama AD, Grusby MJ, Sayegh MH, Khoury SJ
The Journal of clinical investigation 2001 Sep;108(5):739-47
The Journal of clinical investigation 2001 Sep;108(5):739-47
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
Das S, Varalakshmi C, Kumari AL, Patel M, Khar A
Cancer immunology, immunotherapy : CII 2001 Oct;50(8):428-36
Cancer immunology, immunotherapy : CII 2001 Oct;50(8):428-36
CD28 costimulation is required not only to induce IL-12 receptor but also to render janus kinases/STAT4 responsive to IL-12 stimulation in TCR-triggered T cells.
Park WR, Park CS, Tomura M, Ahn HJ, Nakahira Y, Iwasaki M, Gao P, Abe R, Hamaoka T, Fujiwara H
European journal of immunology 2001 May;31(5):1456-64
European journal of immunology 2001 May;31(5):1456-64
CD28-independent induction of experimental autoimmune encephalomyelitis.
Chitnis T, Najafian N, Abdallah KA, Dong V, Yagita H, Sayegh MH, Khoury SJ
The Journal of clinical investigation 2001 Mar;107(5):575-83
The Journal of clinical investigation 2001 Mar;107(5):575-83
Positive regulatory role of IL-12 in macrophages and modulation by IFN-gamma.
Grohmann U, Belladonna ML, Vacca C, Bianchi R, Fallarino F, Orabona C, Fioretti MC, Puccetti P
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul 1;167(1):221-7
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul 1;167(1):221-7
Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice.
Yamamoto N, Suzuki S, Suzuki Y, Shirai A, Nakazawa M, Suzuki M, Takamasu T, Nagashima Y, Minami M, Ishigatsubo Y
Journal of virology 2001 Jan;75(1):499-505
Journal of virology 2001 Jan;75(1):499-505
DNA encoding a single mycobacterial antigen protects against leprosy infection.
Martin E, Roche PW, Triccas JA, Britton WJ
Vaccine 2001 Jan 8;19(11-12):1391-6
Vaccine 2001 Jan 8;19(11-12):1391-6
Dietary ribonucleotides modulate type 1 and type 2 T-helper cell responses against ovalbumin in young BALB/cJ mice.
Jyonouchi H, Sun S, Winship T, Kuchan MJ
The Journal of nutrition 2001 Apr;131(4):1165-70
The Journal of nutrition 2001 Apr;131(4):1165-70
A light, nontoxic interleukin 12 protocol inhibits HER-2/neu mammary carcinogenesis in BALB/c transgenic mice with established hyperplasia.
Cifaldi L, Quaglino E, Di Carlo E, Musiani P, Spadaro M, Lollini PL, Wolf S, Boggio K, Forni G, Cavallo F
Cancer research 2001 Apr 1;61(7):2809-12
Cancer research 2001 Apr 1;61(7):2809-12
Partially TAP-independent protection against Listeria monocytogenes by H2-M3-restricted CD8+ T cells.
Rolph MS, Kaufmann SH
Journal of immunology (Baltimore, Md. : 1950) 2000 Oct 15;165(8):4575-80
Journal of immunology (Baltimore, Md. : 1950) 2000 Oct 15;165(8):4575-80
Tumor vaccination that enhances antitumor T-cell responses does not inhibit the growth of established tumors even in combination with interleukin-12 treatment: the importance of inducing intratumoral T-cell migration.
Gao P, Uekusa Y, Nakajima C, Iwasaki M, Nakahira M, Yang YF, Ono S, Tsujimura T, Fujiwara H, Hamaoka T
Journal of immunotherapy (Hagerstown, Md. : 1997) 2000 Nov-Dec;23(6):643-53
Journal of immunotherapy (Hagerstown, Md. : 1997) 2000 Nov-Dec;23(6):643-53
Mycobacterium bovis bacillus calmette-guérin induces protective immunity against infection by Plasmodium yoelii at blood-stage depending on shifting immunity toward Th1 type and inducing protective IgG2a after the parasite infection.
Matsumoto S, Yukitake H, Kanbara H, Yamada H, Kitamura A, Yamada T
Vaccine 2000 Nov 22;19(7-8):779-87
Vaccine 2000 Nov 22;19(7-8):779-87
Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models.
Iwasaki M, Yu WG, Uekusa Y, Nakajima C, Yang YF, Gao P, Wijesuriya R, Fujiwara H, Hamaoka T
International immunology 2000 May;12(5):701-9
International immunology 2000 May;12(5):701-9
Protection against virulent Mycobacterium avium infection following DNA vaccination with the 35-kilodalton antigen is accompanied by induction of gamma interferon-secreting CD4(+) T cells.
Martin E, Kamath AT, Triccas JA, Britton WJ
Infection and immunity 2000 Jun;68(6):3090-6
Infection and immunity 2000 Jun;68(6):3090-6
Nitric oxide-producing CD11b(+)Ly-6G(Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice.
Angulo I, de las Heras FG, García-Bustos JF, Gargallo D, Muñoz-Fernández MA, Fresno M
Blood 2000 Jan 1;95(1):212-20
Blood 2000 Jan 1;95(1):212-20
Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis.
Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, Sayegh MH, Khoury SJ
The Journal of clinical investigation 2000 Apr;105(8):1109-16
The Journal of clinical investigation 2000 Apr;105(8):1109-16
Cytokine requirements for production of a novel anti-CD8-resistant CTL population.
Lamousé-Smith ES, Dougall DS, McCarthy SA
Journal of immunology (Baltimore, Md. : 1950) 1999 Oct 15;163(8):4160-7
Journal of immunology (Baltimore, Md. : 1950) 1999 Oct 15;163(8):4160-7
The absence of interleukin 1 receptor-related T1/ST2 does not affect T helper cell type 2 development and its effector function.
Hoshino K, Kashiwamura S, Kuribayashi K, Kodama T, Tsujimura T, Nakanishi K, Matsuyama T, Takeda K, Akira S
The Journal of experimental medicine 1999 Nov 15;190(10):1541-8
The Journal of experimental medicine 1999 Nov 15;190(10):1541-8
Structural deficiencies in granuloma formation in TNF gene-targeted mice underlie the heightened susceptibility to aerosol Mycobacterium tuberculosis infection, which is not compensated for by lymphotoxin.
Bean AG, Roach DR, Briscoe H, France MP, Korner H, Sedgwick JD, Britton WJ
Journal of immunology (Baltimore, Md. : 1950) 1999 Mar 15;162(6):3504-11
Journal of immunology (Baltimore, Md. : 1950) 1999 Mar 15;162(6):3504-11
A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system.
Tomura M, Yu WG, Ahn HJ, Yamashita M, Yang YF, Ono S, Hamaoka T, Kawano T, Taniguchi M, Koezuka Y, Fujiwara H
Journal of immunology (Baltimore, Md. : 1950) 1999 Jul 1;163(1):93-101
Journal of immunology (Baltimore, Md. : 1950) 1999 Jul 1;163(1):93-101
Mouse monocyte-derived chemokine is involved in airway hyperreactivity and lung inflammation.
Gonzalo JA, Pan Y, Lloyd CM, Jia GQ, Yu G, Dussault B, Powers CA, Proudfoot AE, Coyle AJ, Gearing D, Gutierrez-Ramos JC
Journal of immunology (Baltimore, Md. : 1950) 1999 Jul 1;163(1):403-11
Journal of immunology (Baltimore, Md. : 1950) 1999 Jul 1;163(1):403-11
Co-immunization with DNA vaccines expressing granulocyte-macrophage colony-stimulating factor and mycobacterial secreted proteins enhances T-cell immunity, but not protective efficacy against Mycobacterium tuberculosis.
Kamath AT, Hanke T, Briscoe H, Britton WJ
Immunology 1999 Apr;96(4):511-6
Immunology 1999 Apr;96(4):511-6
Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis.
Kamath AT, Feng CG, Macdonald M, Briscoe H, Britton WJ
Infection and immunity 1999 Apr;67(4):1702-7
Infection and immunity 1999 Apr;67(4):1702-7
IL-4-independent induction of airway hyperresponsiveness by Th2, but not Th1, cells.
Cohn L, Tepper JS, Bottomly K
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 15;161(8):3813-6
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 15;161(8):3813-6
Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.
Bordmann G, Rudin W, Favre N
Immunology 1998 May;94(1):35-40
Immunology 1998 May;94(1):35-40
A critical role for IL-18 in the proliferation and activation of NK1.1+ CD3- cells.
Tomura M, Zhou XY, Maruo S, Ahn HJ, Hamaoka T, Okamura H, Nakanishi K, Tanimoto T, Kurimoto M, Fujiwara H
Journal of immunology (Baltimore, Md. : 1950) 1998 May 15;160(10):4738-46
Journal of immunology (Baltimore, Md. : 1950) 1998 May 15;160(10):4738-46
Sequential T cell response involved in tumor rejection of sarcoma, Meth A, in syngeneic mice.
Jiao Y, Fujimoto S
Japanese journal of cancer research : Gann 1998 Jun;89(6):657-65
Japanese journal of cancer research : Gann 1998 Jun;89(6):657-65
Endogenous myelin basic protein inactivates the high avidity T cell repertoire.
Targoni OS, Lehmann PV
The Journal of experimental medicine 1998 Jun 15;187(12):2055-63
The Journal of experimental medicine 1998 Jun 15;187(12):2055-63
Topical FK506 suppresses cytokine and costimulatory molecule expression in epidermal and local draining lymph node cells during primary skin immune responses.
Homey B, Assmann T, Vohr HW, Ulrich P, Lauerma AI, Ruzicka T, Lehmann P, Schuppe HC
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 1;160(11):5331-40
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 1;160(11):5331-40
Perforin and IFN-gamma are involved in the antitumor effects of antibody-targeted superantigens.
Rosendahl A, Kristensson K, Hansson J, Riesbeck K, Kalland T, Dohlsten M
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 1;160(11):5309-13
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 1;160(11):5309-13
Cardiac allografts from IL-4 knockout recipients: assessment of transplant arteriosclerosis and peripheral tolerance.
Mottram PL, Räisänen-Sokolowski A, Glysing-Jensen T, Stein-Oakley AN, Russell ME
Journal of immunology (Baltimore, Md. : 1950) 1998 Jul 15;161(2):602-9
Journal of immunology (Baltimore, Md. : 1950) 1998 Jul 15;161(2):602-9
Requirement for distinct Janus kinases and STAT proteins in T cell proliferation versus IFN-gamma production following IL-12 stimulation.
Ahn HJ, Tomura M, Yu WG, Iwasaki M, Park WR, Hamaoka T, Fujiwara H
Journal of immunology (Baltimore, Md. : 1950) 1998 Dec 1;161(11):5893-900
Journal of immunology (Baltimore, Md. : 1950) 1998 Dec 1;161(11):5893-900
B cell-mediated down-regulation of IFN-gamma and IL-12 production induced during anti-tumor immune responses in the tumor-bearing state.
Wijesuriya R, Maruo S, Zou JP, Ogawa M, Umehara K, Yamashita M, Ono S, Fujiwara H, Hamaoka T
International immunology 1998 Aug;10(8):1057-65
International immunology 1998 Aug;10(8):1057-65
Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18.
Tomura M, Maruo S, Mu J, Zhou XY, Ahn HJ, Hamaoka T, Okamura H, Nakanishi K, Clark S, Kurimoto M, Fujiwara H
Journal of immunology (Baltimore, Md. : 1950) 1998 Apr 15;160(8):3759-65
Journal of immunology (Baltimore, Md. : 1950) 1998 Apr 15;160(8):3759-65
A mechanism underlying synergy between IL-12 and IFN-gamma-inducing factor in enhanced production of IFN-gamma.
Ahn HJ, Maruo S, Tomura M, Mu J, Hamaoka T, Nakanishi K, Clark S, Kurimoto M, Okamura H, Fujiwara H
Journal of immunology (Baltimore, Md. : 1950) 1997 Sep 1;159(5):2125-31
Journal of immunology (Baltimore, Md. : 1950) 1997 Sep 1;159(5):2125-31
Humoral and cellular immunity in secondary infection due to murine Chlamydia trachomatis.
Williams DM, Grubbs BG, Pack E, Kelly K, Rank RG
Infection and immunity 1997 Jul;65(7):2876-82
Infection and immunity 1997 Jul;65(7):2876-82
The cytokines IL-4, IFN-gamma, and IL-12 regulate the development of subsets of memory effector helper T cells in vitro.
Bradley LM, Yoshimoto K, Swain SL
Journal of immunology (Baltimore, Md. : 1950) 1995 Aug 15;155(4):1713-24
Journal of immunology (Baltimore, Md. : 1950) 1995 Aug 15;155(4):1713-24
A role for nonspecific (cyclosporin A) or specific (monoclonal antibodies to ICAM-1, LFA-1, and IL-10) immunomodulation in the prolongation of skin allografts after antigen-specific pretransplant immunization or transfusion.
Gorczynski RM, Wojcik D
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 15;152(4):2011-9
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 15;152(4):2011-9
Effects of IL-12 on in vivo cytokine gene expression and Ig isotype selection.
Morris SC, Madden KB, Adamovicz JJ, Gause WC, Hubbard BR, Gately MK, Finkelman FD
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 1;152(3):1047-56
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 1;152(3):1047-56
Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.
Bogen SA, Fogelman I, Abbas AK
Journal of immunology (Baltimore, Md. : 1950) 1993 May 15;150(10):4197-205
Journal of immunology (Baltimore, Md. : 1950) 1993 May 15;150(10):4197-205
Transfected Leishmania expressing biologically active IFN-gamma.
Tobin JF, Reiner SL, Hatam F, Zheng S, Leptak CL, Wirth DF, Locksley RM
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 1;150(11):5059-69
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 1;150(11):5059-69
IFN-gamma involvement in the severity of gram-negative infections in mice.
Kohler J, Heumann D, Garotta G, LeRoy D, Bailat S, Barras C, Baumgartner JD, Glauser MP
Journal of immunology (Baltimore, Md. : 1950) 1993 Jul 15;151(2):916-21
Journal of immunology (Baltimore, Md. : 1950) 1993 Jul 15;151(2):916-21
Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5.
Taguchi T, McGhee JR, Coffman RL, Beagley KW, Eldridge JH, Takatsu K, Kiyono H
Journal of immunology (Baltimore, Md. : 1950) 1990 Jul 1;145(1):68-77
Journal of immunology (Baltimore, Md. : 1950) 1990 Jul 1;145(1):68-77
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFN Gamma was performed by loading 20 µg of indicated whole cell lysates in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IFN Gamma was detected at ~20 kDa using a IFN Gamma rat monoclonal biotin conjugate antibody MM700B at a concentration of 2 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Streptavidin-HRP conjugate (Product # 21126) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFN Gamma was performed by loading indicated amount of Human IFN Gamma recombinant protein in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IFN Gamma was detected at ~16 kDa using a IFN Gamma rat monoclonal biotin conjugate antibody MM700B at a concentration of 2 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Streptavidin-HRP conjugate (Product # 21126) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFN Gamma was performed by loading indicated amount of Human IFN Gamma recombinant protein in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IFN Gamma was detected at ~16 kDa using a IFN Gamma rat monoclonal biotin conjugate antibody MM700B at a concentration of 2 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Streptavidin-HRP conjugate (Product # 21126) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of IFN gamma was performed by coating wells of a 96-well plate with 100 µL per well of Human IFN gamma (Product # RIFNG100) diluted in carbonate/bicarbonate buffer (Product # 28382), starting at a concentration of 1000pg/mL and serially diluting 2-fold to a concentration of 1 µg/mL, overnight at 4°C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse anti-IFN gamma Biotin conjugated monoclonal antibody (Product # MM700B) at a concentration of 1 µg/mL for 1 hour at 37C. The plate was washed, then incubated with 100 µL per well of an streptavidin HRP-conjugate (Product # 21126) at a dilution of 1:5000 for 1 hour at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA of IFN gamma was performed by coating wells of a 96-well plate with 100 µL of an IFN gamma mouse monoclonal antibody (Product # MM700) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382) overnight at 4°C. Wells were blocked with 200 µL of StartingBlock T20 (TBS) Blocking Buffer (Product # 28382) for 2 hours, and 100 µL of recombinant mouse IFN gamma protein ranging from 16-3000 pg/mL were incubated for 1 hour at room temperature. The plate was washed with 1X PBS (Product # 28348), and 100 µL per well of an IFN gamma Biotin conjugated mouse monoclonal antibody (Product # MM700B) diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100 µL per well of a Streptavidin-HRP (Product # 21126) was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 15-30 minutes, followed by Stop Solution (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Corylin improves the TLR4 signal pathway in DSS-induced colitis mice. ( A ) Expression analysis by immunohistochemical staining of TLR4, p-p38, and AP-1 in mice after the indicated treatment. Scale bar: 100 mum. Red arrows highlight positive staining. ( B ) Western blot analysis of TLR4, MyD88, p-p38, AP-1, and beta-actin (loading control) in colon homogenates. Right graph indicates quantification relative to beta-actin. ( C , D ) mRNA expression of Ifngamma , Tnf-alpha , Il-6 , Il-1beta , Nlrp3 , Asc , Pannexin , and Pro-caspase 1 determined by qRT-PCR. ( E , F ) Western blot analysis of IFNgamma, TNF-alpha, IL-6, IL-1beta, NLRP3, ASC, Pannexin, and Caspase 1, and beta-actin (loading control) in colon homogenates. Results represent mean +- SEM. * p < 0.05, Normal compared with DSS; # p < 0.05, DSS compared with DSS + Corylin (L); Y= p < 0.05, DSS compared with DSS + Corylin (H); SS p < 0.05, DSS + Corylin (L) compared with DSS + Corylin (H). DSS, dextran sodium sulfate; TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response 88; AP-1, activator protein 1; Ifngamma , interferon gamma; Tnf-alpha , tumor necrosis factor-alpha; Il-6 , interleukin-6; Nlrp3 , NACHT, LRR, and PYD domains-containing protein 3.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Corylin improves the TLR4 signaling pathway in AOM/DSS-induced-colitis-associated colorectal cancer mice. ( A ) Expression analysis by immunohistochemical staining of TLR4, p-p38, and AP-1 in mice after the indicated treatment. Scale bar: 100 mum. ( B ) Western blot analysis of TLR4, MyD88, p-p38, AP-1, and beta-actin (loading control) in colon. ( C , D ) mRNA expression of Ifngamma , Tnf-alpha , Il-6 , Il-1beta , Nlrp3 , Asc , Pannexin , and Pro-caspase 1 as determined by qRT-PCR. ( E , F ) Western blot analysis of IFNgamma, TNF-alpha, IL-6, IL-1beta, NLRP3, ASC, Pannexin, and Caspase 1, and beta-actin (loading control) in colon homogenates. Results represent mean +- SEM. * p < 0.05, Normal compared with AOM/DSS; # p < 0.05, AOM/DSS compared with AOM/DSS + Corylin (L); Y= p < 0.05, AOM/DSS compared with AOM/DSS + Corylin (H); SS p < 0.05, DSS + Corylin (L) compared with DSS + Corylin (H). AOM, azoxymethane; DSS, dextran sodium sulfate; TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response 88; AP-1, activator protein 1; Ifngamma , interferon gamma; Tnf-alpha , tumor necrosis factor-alpha; Il-6 , interleukin-6; Nlrp3 , NACHT, LRR, and PYD domains-containing protein 3.
- Conjugate
- Biotin
