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- Antibody Data
- Antigen structure
- References [18]
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- Product number
- 14-7317-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFN gamma Monoclonal Antibody (MD-1), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN gamma), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN gamma is a dimeric protein. IFN gamma has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells. Applications Reported: The MD-1 antibody has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA, and ELISPOT. (Fluorochrome conjugated MD-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified MD-1, Product # 16-7317, in functional assays). Applications Tested: The MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon gamma (IFN gamma) in combination with the biotinylated 4S.B3 (Product # 13-7319-81) antibody for detection and recombinant human IFN gamma (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN gamma biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/mL. For in vitro neutralization, the MD-1 antibody at 30 µg/mL has been found to neutralize by 50% the effect of 1 ng/mL human IFN gamma. A more potent neutralizing antibody is clone NIB42. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MD-1
- Vial size
- 500 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap.
Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.
IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.
MAIT cells are activated during human viral infections.
Targeted Vaccination against Human α-Lactalbumin for Immunotherapy and Primary Immunoprevention of Triple Negative Breast Cancer.
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vγ2Vδ2 T cells after Mycobacterium tuberculosis infection or vaccination.
Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.
Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis.
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis.
Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis.
Immediate cytotoxicity but not degranulation distinguishes effector and memory subsets of CD8+ T cells.
Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients.
Enumeration of lymphokine-secreting cells as a quantitative measure for cellular immune responses in rhesus macaques.
The mitogen-induced generation of interferon-gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age-dependent.
Elevated levels of T cell activation antigen CD27 and increased interleukin-4 production in human lymphatic filariasis.
Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA.
Bounab Y, Eyer K, Dixneuf S, Rybczynska M, Chauvel C, Mistretta M, Tran T, Aymerich N, Chenon G, Llitjos JF, Venet F, Monneret G, Gillespie IA, Cortez P, Moucadel V, Pachot A, Troesch A, Leissner P, Textoris J, Bibette J, Guyard C, Baudry J, Griffiths AD, Védrine C
Nature protocols 2020 Sep;15(9):2920-2955
Nature protocols 2020 Sep;15(9):2920-2955
Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.
Lee JY, Hall JA, Kroehling L, Wu L, Najar T, Nguyen HH, Lin WY, Yeung ST, Silva HM, Li D, Hine A, Loke P, Hudesman D, Martin JC, Kenigsberg E, Merad M, Khanna KM, Littman DR
Cell 2020 Jan 9;180(1):79-91.e16
Cell 2020 Jan 9;180(1):79-91.e16
IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.
Zhao M, Tan Y, Peng Q, Huang C, Guo Y, Liang G, Zhu B, Huang Y, Liu A, Wang Z, Li M, Gao X, Wu R, Wu H, Long H, Lu Q
Nature communications 2018 Feb 8;9(1):583
Nature communications 2018 Feb 8;9(1):583
MAIT cells are activated during human viral infections.
van Wilgenburg B, Scherwitzl I, Hutchinson EC, Leng T, Kurioka A, Kulicke C, de Lara C, Cole S, Vasanawathana S, Limpitikul W, Malasit P, Young D, Denney L, STOP-HCV consortium, Moore MD, Fabris P, Giordani MT, Oo YH, Laidlaw SM, Dustin LB, Ho LP, Thompson FM, Ramamurthy N, Mongkolsapaya J, Willberg CB, Screaton GR, Klenerman P
Nature communications 2016 Jun 23;7:11653
Nature communications 2016 Jun 23;7:11653
Targeted Vaccination against Human α-Lactalbumin for Immunotherapy and Primary Immunoprevention of Triple Negative Breast Cancer.
Tuohy VK, Jaini R, Johnson JM, Loya MG, Wilk D, Downs-Kelly E, Mazumder S
Cancers 2016 Jun 16;8(6)
Cancers 2016 Jun 16;8(6)
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vγ2Vδ2 T cells after Mycobacterium tuberculosis infection or vaccination.
Shen H, Wang Y, Chen CY, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen ZW
European journal of immunology 2015 Feb;45(2):442-51
European journal of immunology 2015 Feb;45(2):442-51
Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.
Price JV, Haddon DJ, Kemmer D, Delepine G, Mandelbaum G, Jarrell JA, Gupta R, Balboni I, Chakravarty EF, Sokolove J, Shum AK, Anderson MS, Cheng MH, Robinson WH, Browne SK, Holland SM, Baechler EC, Utz PJ
The Journal of clinical investigation 2013 Dec;123(12):5135-45
The Journal of clinical investigation 2013 Dec;123(12):5135-45
Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K
PloS one 2012;7(5):e37220
PloS one 2012;7(5):e37220
Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis.
Yao S, Huang D, Chen CY, Halliday L, Zeng G, Wang RC, Chen ZW
PLoS pathogens 2010 Feb 26;6(2):e1000789
PLoS pathogens 2010 Feb 26;6(2):e1000789
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
Gong G, Shao L, Wang Y, Chen CY, Huang D, Yao S, Zhan X, Sicard H, Wang R, Chen ZW
Blood 2009 Jan 22;113(4):837-45
Blood 2009 Jan 22;113(4):837-45
Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis.
Jurado JO, Alvarez IB, Pasquinelli V, Martínez GJ, Quiroga MF, Abbate E, Musella RM, Chuluyan HE, García VE
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):116-25
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):116-25
Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis.
Jurado JO, Alvarez IB, Pasquinelli V, Martínez GJ, Quiroga MF, Abbate E, Musella RM, Chuluyan HE, García VE
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):116-25
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):116-25
Immediate cytotoxicity but not degranulation distinguishes effector and memory subsets of CD8+ T cells.
Wolint P, Betts MR, Koup RA, Oxenius A
The Journal of experimental medicine 2004 Apr 5;199(7):925-36
The Journal of experimental medicine 2004 Apr 5;199(7):925-36
Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients.
Wassenaar A, Reinhardus C, Thepen T, Abraham-Inpijn L, Kievits F
Infection and immunity 1995 Jun;63(6):2147-53
Infection and immunity 1995 Jun;63(6):2147-53
Enumeration of lymphokine-secreting cells as a quantitative measure for cellular immune responses in rhesus macaques.
van der Meide PH, Groenestein RJ, de Labie MC, Heeney J, Pala P, Slaoui M
Journal of medical primatology 1995 Dec;24(4):271-81
Journal of medical primatology 1995 Dec;24(4):271-81
The mitogen-induced generation of interferon-gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age-dependent.
van Besouw NM, van der Meide PH, Bakker NP
Journal of medical primatology 1994 Jan;23(1):42-8
Journal of medical primatology 1994 Jan;23(1):42-8
Elevated levels of T cell activation antigen CD27 and increased interleukin-4 production in human lymphatic filariasis.
Yazdanbakhsh M, Sartono E, Kruize YC, Kurniawan A, van der Pouw-Kraan T, van der Meide PH, Selkirk ME, Partono F, Hintzen RQ, van Lier RA
European journal of immunology 1993 Dec;23(12):3312-7
European journal of immunology 1993 Dec;23(12):3312-7
Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA.
Van der Meide PH, Dubbeld M, Schellekens H
Journal of immunological methods 1985 May 23;79(2):293-305
Journal of immunological methods 1985 May 23;79(2):293-305
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human PBMCs were activated with PMA/Ionomycin in Human IFN gamma ELISPOT assay. Control well is medium alone.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
