Antibody data

Product number
606-7319-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor™ 660, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity. Applications Reported: This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4S.B3 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700. Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
4S.B3
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
IFNG protein structure - 606-7319-41 shown in red.