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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-285-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant rhesus monkey IFN-gamma is observed, and less than 5% cross-reactivity with recombinant mouse IFN-gamma , recombinant canine IFN-gamma , recombinant porcine IFN-gamma , recombinant bovine IFN-gamma , recombinant rat IFN-gamma , recombinant feline IFN-gamma , and recombinant equine IFN-gamma is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14609- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
Journal of immunology research 2014;2014:593562
Journal of immunology research 2014;2014:593562
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-gamma Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma Poly-clonal Antibody (Catalog # AB-285-NA). The ND50 is typically 1-5 µg/mL.
