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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-285-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant rat IFN-gamma and recombinant mouse IFN-gamma is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14609- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
Natural killer cell-produced IFN-γ and TNF-α induce target cell cytolysis through up-regulation of ICAM-1.
Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-γ.
Immunoproteasome expression is induced in mesial temporal lobe epilepsy.
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Upregulated IL-23 and IL-17 in Behçet patients with active uveitis.
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
Zheng J, Wu WL, Liu Y, Xiang Z, Liu M, Chan KH, Lau SY, Lam KT, To KK, Chan JF, Li L, Chen H, Lau YL, Yuen KY, Tu W
PloS one 2015;10(8):e0135999
PloS one 2015;10(8):e0135999
Natural killer cell-produced IFN-γ and TNF-α induce target cell cytolysis through up-regulation of ICAM-1.
Wang R, Jaw JJ, Stutzman NC, Zou Z, Sun PD
Journal of leukocyte biology 2012 Feb;91(2):299-309
Journal of leukocyte biology 2012 Feb;91(2):299-309
Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-γ.
Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
PLoS pathogens 2011 Apr;7(4):e1002016
PLoS pathogens 2011 Apr;7(4):e1002016
Immunoproteasome expression is induced in mesial temporal lobe epilepsy.
Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C
Biochemical and biophysical research communications 2011 Apr 29;408(1):65-70
Biochemical and biophysical research communications 2011 Apr 29;408(1):65-70
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
Blood 2010 Sep 23;116(12):2061-9
Blood 2010 Sep 23;116(12):2061-9
IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
Immunology letters 2010 Jan 4;127(2):150-6
Immunology letters 2010 Jan 4;127(2):150-6
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
Mucosal immunology 2008 Sep;1(5):412-20
Mucosal immunology 2008 Sep;1(5):412-20
Upregulated IL-23 and IL-17 in Behçet patients with active uveitis.
Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
Investigative ophthalmology & visual science 2008 Jul;49(7):3058-64
Investigative ophthalmology & visual science 2008 Jul;49(7):3058-64
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
European journal of immunology 2008 Apr;38(4):1012-23
European journal of immunology 2008 Apr;38(4):1012-23
Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
Hessian PA, Highton J, Kean A, Sun CK, Chin M
Arthritis and rheumatism 2003 Feb;48(2):334-8
Arthritis and rheumatism 2003 Feb;48(2):334-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-gamma Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND50 is typically 0.1-0.3 µg/mL.
