Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- EB05040 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB05040, RRID:AB_2302281
- Product name
- Goat anti-SOCS1 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-SOCS1 Antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
C-VLRDYLSSFPFQI
- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 overexpressing SOCS1 and probed with EB05040 (mock transfection in lane B).
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB05040 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB05040 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.