F51354

antibody from NSJ Bioreagents

Targeting: SOCS1 Cish1, JAB, SOCS-1, SSI-1, TIP3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F51354

Provider

NSJ Bioreagents

Product name

SOCS1 Antibody / TIP3 / SSI1

Antibody type

Polyclonal

Description

This highly specific SOCS1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the SOCS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of SOCS1 antibody at 1:2000 dilution. Lane 1: mouse thymus lysate; 2: rat spleen lysate; Predicted molecular weight ~ 24 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of SOCS1 antibody and mouse kidney tissue lysate. Predicted molecular weight ~ 24 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of SOCS1 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human colon carcinoma stained with SOCS1 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SOCS1 antibody flow cytometric analysis of WiDr cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.