MAB4175

antibody from Novus Biologicals

Targeting: PRKACA PKACa

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4175

Provider

Novus Biologicals

Product name

Mouse Monoclonal PKA C (pan) Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human PKA C alpha , C beta , and C gamma as well as endogenous human, mouse, and rat PKA C isoforms at 39-42 kDa using Western blot.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Neuromedin B regulates steroidogenesis, cell viability and apoptosis in rabbit Leydig cells.

Ma Z, Guo J, Zhang Y, Zhang Y, Zhang M, Zong R, Chen F, Zhang J
General and comparative endocrinology 2020 Mar 1;288:113371

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat PKA C by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.