H00005566-M02

antibody from Abnova Corporation

Targeting: PRKACA PKACa

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005566-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005566-M02, RRID:AB_1112052

Product name

PRKACA monoclonal antibody (M02), clone 1D7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PRKACA.

Antigen sequence

MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPA
QNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHY
AMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLV
KLEFSFKDNSNLYMV


Isotype

IgG

Antibody clone number

1D7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PRKACA is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAP3K3 and PRKACA. HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-PRKACA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)