Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [1]
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Validation data
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- Product number
- MAB3591 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3591, RRID:AB_10554674
- Product name
- PRKACA monoclonal antibody, clone 6D2.1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of PRKACA.
- Antigen sequence
ESPAQNTAHLDQFERIK
- Isotype
- IgG
- Antibody clone number
- 6D2.1
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references New faces of the familiar clathrin lattice.
Cargo- and compartment-selective endocytic scaffold proteins.
Monoclonal antibody BF-06 against the heavy chain of clathrin.
Wilbur JD, Hwang PK, Brodsky FM
Traffic (Copenhagen, Denmark) 2005 Apr;6(4):346-50
Traffic (Copenhagen, Denmark) 2005 Apr;6(4):346-50
Cargo- and compartment-selective endocytic scaffold proteins.
Szymkiewicz I, Shupliakov O, Dikic I
The Biochemical journal 2004 Oct 1;383(Pt 1):1-11
The Biochemical journal 2004 Oct 1;383(Pt 1):1-11
Monoclonal antibody BF-06 against the heavy chain of clathrin.
Macůrek L, Zíková M, Dráberová E, Dráber P
Folia biologica 2003;49(6):238-40
Folia biologica 2003;49(6):238-40
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 (Cat # MAB3591) and GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1 : GST-Akt1.Lane 2 : GST-Akt2.Lane 3 : GST-Akt3.Lane 4 : GST-PKAc alpha.Lane 5 : GST-PKAc beta.Lane 6 : GST-PKAc gamma.Lane 7 : GST-MEK 1.Lane 8 : GST.
- Validation comment
- Dot Blot