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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-30189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AP2 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7, rat brain.
- Concentration
- 0.95 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AP2 beta was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AP2 beta Polyclonal Antibody (Product # PA5-30189) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AP2 beta Polyclonal Antibody (Product # PA5-30189). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 10% SDS PAGE. AP2 beta Polyclonal Antibody (Product # PA5-30189) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AP2 beta Polyclonal Antibody immunoprecipitates AP2 beta protein in IP experiments. IP samples: MCF-7 whole cell extract. A. 30 µg MCF-7 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of AP2 beta protein by 4 µg AP2 beta Polyclonal Antibody (Product # PA5-30189). 7.5 % SDS-PAGE. The immunoprecipitated AP2 beta protein was detected by AP2 beta Polyclonal Antibody (Product # PA5-30189) diluted at 1:1,000.
