Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-22310 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HNF1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, Mouse liver, Rat liver.
- Concentration
- 1.27 mg/mL
Submitted references Lack of TMEM27 expression is associated with postoperative progression of clinically localized conventional renal cell carcinoma.
Javorhazy A, Farkas N, Beothe T, Pusztai C, Szanto A, Kovacs G
Journal of cancer research and clinical oncology 2016 Sep;142(9):1947-53
Journal of cancer research and clinical oncology 2016 Sep;142(9):1947-53
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HNF-1 alpha using 30 µg HepG2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HNF-1 alpha polyclonal antibody (Product # PA5-22310) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HNF1 alpha in Mouse tissue extracts (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with HNF1 alpha Polyclonal antibody (Product # PA5-22310) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HNF1A Polyclonal Antibody detects HNF1 alpha protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HNF1A Polyclonal Antibody detects HNF1 alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HNF1A was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1000. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HNF1A was performed in paraffin-embedded mouse colon tissue using HNF1A Polyclonal Antibody (Product # PA5-22310) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HNF1A was performed in paraffin-embedded mouse intestine tissue using HNF1A Polyclonal Antibody (Product # PA5-22310) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha (Product # PA5-22310) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.