PA5-22310

antibody from Invitrogen Antibodies

Targeting: HNF1A HNF1, LFB1, MODY3, TCF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22310

Provider

Invitrogen Antibodies

Product name

HNF1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, Mouse liver, Rat liver. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (83%), Pig (99%), Chicken (95%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.27 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lack of TMEM27 expression is associated with postoperative progression of clinically localized conventional renal cell carcinoma.

Javorhazy A, Farkas N, Beothe T, Pusztai C, Szanto A, Kovacs G
Journal of cancer research and clinical oncology 2016 Sep;142(9):1947-53

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HNF-1 alpha using 30 µg HepG2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HNF-1 alpha polyclonal antibody (Product # PA5-22310) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HNF1 alpha in Mouse tissue extracts (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with HNF1 alpha Polyclonal antibody (Product # PA5-22310) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HNF1A Polyclonal Antibody detects HNF1 alpha protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HNF1A Polyclonal Antibody detects HNF1 alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HNF1A was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1000. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of HNF1A was performed in paraffin-embedded mouse colon tissue using HNF1A Polyclonal Antibody (Product # PA5-22310) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of HNF1A was performed in paraffin-embedded mouse intestine tissue using HNF1A Polyclonal Antibody (Product # PA5-22310) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha (Product # PA5-22310) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.