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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00004354-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004354-M01, RRID:AB_425553
- Product name
- MPP1 monoclonal antibody (M01), clone 1E11-1G11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MPP1.
- Antigen sequence
MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEA
VSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVR
LIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMI
HRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKET
KGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDN
LIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGS
SKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPF
GKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAF
KRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVP
YTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLE
FGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTL
KIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSE
AIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACS
SPQWVPVSWVY- Isotype
- IgG
- Antibody clone number
- 1E11-1G11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MPP1 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
