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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PB10078 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MPP1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for MPP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. MPP1 information: Molecular Weight: 52296 MW; Subcellular Localization: Membrane; Lipid-anchor. Cell projection, stereocilium . Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non- myelin-forming Schwann cells; Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Inositol-requiring enzyme 1α links palmitate-induced mTOR activation and lipotoxicity in hepatocytes.
Chen Y, Griffiths A, Wang J, Zhang T, Song Q, Song Z
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1130-C1140
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1130-C1140
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of MPP1 expression in rat lung extract (lane 1), mouse spleen extract (lane 2) and MCF-7 whole cell lysates (lane 3). MPP1 at 55KD was detected using rabbit anti- MPP1 Antigen Affinity purified polyclonal antibody (Catalog # PB10078) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image
