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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00004354-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004354-M01, RRID:AB_540572
- Product name
- Mouse Monoclonal MPP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MPP1 - membrane protein, palmitoylated 1, 55kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The microdomain-organizing protein MPP1 is required for insulin-stimulated activation of H-Ras.
Podkalicka J, Biernatowska A, Olszewska P, Tabaczar S, Sikorski AF
Oncotarget 2018 Apr 6;9(26):18410-18421
Oncotarget 2018 Apr 6;9(26):18410-18421
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - MPP1 monoclonal antibody (M01), clone 1E11-1G11. Analysis of MPP1 expression in human placenta.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of monoclonal antibody to MPP1 on HeLa cell . Antibody concentration 10 ug/ml.
