Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PB9535 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cdk4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CDK4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CDK4 information: Molecular Weight: 33730 MW; Subcellular Localization: Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Icariside I reduces breast cancer proliferation, apoptosis, invasion, and metastasis probably through inhibiting IL-6/STAT3 signaling pathway.
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer.
Targeted p21 activation by a new double stranded RNA suppresses human prostate cancer cells growth and metastasis.
Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.
Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone.
Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway.
JNK/STAT signalling pathway is involved in fluoride-induced follicular developmental dysplasia in female mice.
OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells.
Wall shear stress promotes intimal hyperplasia through the paracrine H(2)O(2)-mediated NOX-AKT-SVV axis.
Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells.
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene.
Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells.
The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats.
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins.
Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells.
Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo.
Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats.
Immunoregulatory and anti-tumor effects of polysaccharopeptide and Astragalus polysaccharides on tumor-bearing mice.
Hou M, Li H, He T, Hui S, Dai W, Hou X, Zhao J, Zhao J, Wen J, Kan W, Xiao X, Zhan X, Bai Z
The Journal of pharmacy and pharmacology 2024 May 3;76(5):499-513
The Journal of pharmacy and pharmacology 2024 May 3;76(5):499-513
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Sun M, Zhou D, Wu J, Zhou J, Xu J
Marine drugs 2022 Feb 5;20(2)
Marine drugs 2022 Feb 5;20(2)
Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer.
Lu Z, Song W, Zhang Y, Wu C, Zhu M, Wang H, Li N, Zhou Y, Xu H
Frontiers in oncology 2021;11:648985
Frontiers in oncology 2021;11:648985
Targeted p21 activation by a new double stranded RNA suppresses human prostate cancer cells growth and metastasis.
Zhang Q, Yang X, Luo L, Ma X, Jiao W, Li B, Zhang M, Zhao K, Niu H
American journal of translational research 2020;12(8):4175-4188
American journal of translational research 2020;12(8):4175-4188
Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.
Wu Y, Ma J, Sun Y, Tang M, Kong L
Chemosphere 2020 Sep;255:126913
Chemosphere 2020 Sep;255:126913
Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone.
Yin F, Zhao L, Zhang L, Chen Y, Sun G, Li J, Zhang N, Xu Y, Chan PK, Zhong R
Biomedicines 2020 Nov 4;8(11)
Biomedicines 2020 Nov 4;8(11)
Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway.
Fan SH, Xiong QF, Wang L, Zhang LH, Shi YW
Molecular and cellular endocrinology 2020 Dec 1;518:111005
Molecular and cellular endocrinology 2020 Dec 1;518:111005
JNK/STAT signalling pathway is involved in fluoride-induced follicular developmental dysplasia in female mice.
Zhao WP, Wang HW, Liu J, Tan PP, Lin L, Zhou BH
Chemosphere 2018 Oct;209:88-95
Chemosphere 2018 Oct;209:88-95
OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells.
Ren J, Liu Y, Li L, Zhao Y, Li Z, Wu C, Chen L, Hu K
Cell biology international 2018 Feb;42(2):194-204
Cell biology international 2018 Feb;42(2):194-204
Wall shear stress promotes intimal hyperplasia through the paracrine H(2)O(2)-mediated NOX-AKT-SVV axis.
Zhang H, Yang Z, Wang J, Wang X, Zhao Y, Zhu F
Life sciences 2018 Aug 15;207:61-71
Life sciences 2018 Aug 15;207:61-71
Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells.
Li C, Ge Q, Liu J, Zhang Q, Wang C, Cui K, Chen Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Jun;39(6):1010428317710824
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Jun;39(6):1010428317710824
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene.
Kong Y, Lu ZL, Wang JJ, Zhou R, Guo J, Liu J, Sun HL, Wang H, Song W, Yang J, Xu HX
Oncology reports 2016 Sep;36(3):1447-56
Oncology reports 2016 Sep;36(3):1447-56
Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells.
Zhang H, Zhao B, Huang C, Meng XM, Bian EB, Li J
PloS one 2014;9(5):e95520
PloS one 2014;9(5):e95520
The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats.
Wang HY, Huang RP, Han P, Xue DB, Li HB, Liu B, Shan P, Wang QS, Li KS, Li HL
Cell biochemistry and function 2014 Mar;32(2):201-8
Cell biochemistry and function 2014 Mar;32(2):201-8
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins.
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X
Biochemical and biophysical research communications 2013 Oct 18;440(2):322-9
Biochemical and biophysical research communications 2013 Oct 18;440(2):322-9
Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells.
Zhang J, Ge Y, Sun L, Cao J, Wu Q, Guo L, Wang Z
International journal of medical sciences 2012;9(2):184-92
International journal of medical sciences 2012;9(2):184-92
Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo.
Li BH, Xu SB, Li F, Zou XG, Saimaiti A, Simayi D, Wang YH, Zhang Y, Yuan J, Zhang WJ
Cellular signalling 2012 Mar;24(3):718-25
Cellular signalling 2012 Mar;24(3):718-25
Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats.
Cai K, Di Q, Shi J, Zhang Y
Neuroscience letters 2009 Dec 18;467(1):15-9
Neuroscience letters 2009 Dec 18;467(1):15-9
Immunoregulatory and anti-tumor effects of polysaccharopeptide and Astragalus polysaccharides on tumor-bearing mice.
Li J, Bao Y, Lam W, Li W, Lu F, Zhu X, Liu J, Wang H
Immunopharmacology and immunotoxicology 2008;30(4):771-82
Immunopharmacology and immunotoxicology 2008;30(4):771-82
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti- Cdk4 Picoband antibody, PB9535, Western blottingAll lanes: Anti Cdk4 (PB9535) at 0.5ug/ml Lane 1: Rat Gaster Tissue Lysate at 50ug Lane 2: Rat Thymus Tissue Lysate at 50ug Lane 3: HELA Whole Cell Lysate at 40ug Lane 4: A549 Whole Cell Lysate at 40ug Lane 5: SKOV Whole Cell Lysate at 40ug Lane 6: 22RV1 Whole Cell Lysate at 40ug Predicted bind size: 34KD Observed bind size: 34KD
- Additional image