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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- PB9535 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cdk4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CDK4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CDK4 information: Molecular Weight: 33730 MW; Subcellular Localization: Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti- Cdk4 Picoband antibody, PB9535, Western blottingAll lanes: Anti Cdk4 (PB9535) at 0.5ug/ml Lane 1: Rat Gaster Tissue Lysate at 50ug Lane 2: Rat Thymus Tissue Lysate at 50ug Lane 3: HELA Whole Cell Lysate at 40ug Lane 4: A549 Whole Cell Lysate at 40ug Lane 5: SKOV Whole Cell Lysate at 40ug Lane 6: 22RV1 Whole Cell Lysate at 40ug Predicted bind size: 34KD Observed bind size: 34KD
- Additional image
