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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA321050 - Provider product page
- Provider
- OriGene
- Product name
- Anti-CDK4 Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Anti-CDK4 Rabbit Polyclonal Antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 0.9mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 34kDa. Positive control: Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lysates: 30 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm, Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CDK4 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200)
- Validation comment
- IHC
