LS-C496617

antibody from LSBio

Targeting: CDK4 PSK-J3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C496617

Provider

LSBio

Product name

CDK4 Antibody LS-C496617

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Storage

Store at 4°C, avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of HepG2 cell and HeLa cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.