H00001019-M10

antibody from Abnova Corporation

Targeting: CDK4 PSK-J3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001019-M10

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001019-M10, RRID:AB_1572479

Product name

CDK4 monoclonal antibody (M10), clone 1E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant CDK4.

Antigen sequence

MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALK
SVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPN
VVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLD
KAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRD
LKPENILVTSGGTVKLADFGLARIYSYQMALTPVV
VTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRR
KPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLP
RGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNP
HKRISAFRALQHSYLHKDEGNPE


Isotype

IgG

Antibody clone number

1E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M10), clone 1E1.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CDK4 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol