711586

antibody from Invitrogen Antibodies

Targeting: CDK4 PSK-J3

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711586

Provider

Invitrogen Antibodies

Product name

Phospho-CDK4 (Thr172) Recombinant Polyclonal Antibody (9HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

9HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, T98G cells were fixed and permeabilized for detection of endogenous CDK4 (pT172) using Anti- CDK4 (pT172) Recombinant Rabbit Polyclonal Antibody (Product # 711586, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of CDK4 pT172 (green) in the nucleus can be clearly observed in cell cycle synchronised cells (serum starved overnight and serum released for 10 hours) as compared to untreated cells. Antibody specificity was demonstrated by competition with the CDK4 pT172 peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.