Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN359662 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Cyclin-Dependent Kinase 4 (CDK4) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the C-terminal region of human CDK4.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Submitted references Cyclins and cyclin-dependent kinases: comparative study of hepatocellular carcinoma versus cirrhosis.
Bypass of telomere-dependent replicative senescence (M1) upon overexpression of Cdk4 in normal human epithelial cells.
Downregulation of p21(waf/cip-1) mediates apoptosis of human hepatocellular carcinoma cells in response to interferon-gamma.
Phosphorylation of p16INK4A correlates with Cdk4 association.
Absence of R24C mutation of the CDK4 gene in leukemias and solid tumors.
Masaki T, Shiratori Y, Rengifo W, Igarashi K, Yamagata M, Kurokohchi K, Uchida N, Miyauchi Y, Yoshiji H, Watanabe S, Omata M, Kuriyama S
Hepatology (Baltimore, Md.) 2003 Mar;37(3):534-43
Hepatology (Baltimore, Md.) 2003 Mar;37(3):534-43
Bypass of telomere-dependent replicative senescence (M1) upon overexpression of Cdk4 in normal human epithelial cells.
Ramirez RD, Herbert BS, Vaughan MB, Zou Y, Gandia K, Morales CP, Wright WE, Shay JW
Oncogene 2003 Jan 23;22(3):433-44
Oncogene 2003 Jan 23;22(3):433-44
Downregulation of p21(waf/cip-1) mediates apoptosis of human hepatocellular carcinoma cells in response to interferon-gamma.
Detjen KM, Murphy D, Welzel M, Farwig K, Wiedenmann B, Rosewicz S
Experimental cell research 2003 Jan 15;282(2):78-89
Experimental cell research 2003 Jan 15;282(2):78-89
Phosphorylation of p16INK4A correlates with Cdk4 association.
Gump J, Stokoe D, McCormick F
The Journal of biological chemistry 2003 Feb 28;278(9):6619-22
The Journal of biological chemistry 2003 Feb 28;278(9):6619-22
Absence of R24C mutation of the CDK4 gene in leukemias and solid tumors.
Mori N, Yang R, Kawamata N, Miller CW, Mizoguchi H, Koeffler HP
International journal of hematology 2003 Apr;77(3):259-62
International journal of hematology 2003 Apr;77(3):259-62
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-CDK4 Pab in HL-60 cell lysate. CDK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
