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- Antibody Data
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- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-80462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human CDK4.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Simultaneous detection of multiple mRNAs and proteins in bovine IVD cells and tissue with single cell resolution.
Li K, Varden L, Henderson A, Lufkin T, Kraus P
Biotechnology letters 2021 Jan;43(1):13-24
Biotechnology letters 2021 Jan;43(1):13-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of CDK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CDK4 Polyclonal Antibody (Product # PA5-80462, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of CDK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CDK4 Polyclonal Antibody (Product # PA5-80462, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDK4 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg k562 Whole Cell Lysate, Lane C: 0.5 mg Hela Whole Cell Lysate 2 µL with CDK4 Polyclonal Antibody (Product # PA5-80462) and 15 µL of 50 % Protein G agarose. Primary antibody: CDK4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CDK4 in human breast carcinoma with CDK4 Polyclonal Antibody (Product # PA5-80462, 1:1,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDK4 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg k562 Whole Cell Lysate, Lane C: 0.5 mg Hela Whole Cell Lysate 2 µL with CDK4 Polyclonal Antibody (Product # PA5-80462) and 15 µL of 50 % Protein G agarose. Primary antibody: CDK4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.
