NBP1-31308

antibody from Novus Biologicals

Targeting: CDK4 PSK-J3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31308

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31308, RRID:AB_2078700

Product name

Rabbit Polyclonal CDK4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Feline

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Prostaglandin F_2α-PTGFR signaling promotes proliferation of endometrial epithelial cells of cattle through cell cycle regulation.

Fu C, Mao W, Gao R, Deng Y, Gao L, Wu J, Zhang S, Shen Y, Liu K, Li Q, Song X, Cao J, Liu B
Animal reproduction science 2020 Feb;213:106276

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Proteins of the retinoblastoma pathway, FEN1 and MGMT are novel potential prognostic biomarkers in pancreatic adenocarcinoma.

Isohookana J, Haapasaari KM, Soini Y, Leppänen J, Karihtala P
Pathology, research and practice 2018 Jun;214(6):840-847

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Cell cycle S phase markers are expressed in cerebral neuron nuclei of cats infected by the Feline Panleukopenia Virus.

Poncelet L, Garigliany M, Ando K, Franssen M, Desmecht D, Brion JP
Cell cycle (Georgetown, Tex.) 2016 Dec 16;15(24):3482-3489

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UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.

Huang AM, Kao YT, Toh S, Lin PY, Chou CH, Hu HT, Lu CY, Liou JY, Chao SY, Hour TC, Pu YS
Biochemical pharmacology 2011 Jul 1;82(1):35-42

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cdk4 Antibody [NBP1-31308] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cdk4 Antibody [NBP1-31308] - Paraffin-embedded Gastric ca, using antibody at 1:500 dilution.