PA5-14445

antibody from Invitrogen Antibodies

Targeting: CDK4 PSK-J3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14445

Provider

Invitrogen Antibodies

Product name

CDK4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a CDK4 polyclonal antibody (Product # PA5-14445). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a CDK4 polyclonal antibody (Product # PA5-14445) (1:25, 1 hr at 37ºC). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37ºC). Actin filaments have been labeled with dye-conjugated phalloidin (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of CDK4 in HeLa cells. Samples were incubated with CDK4 polyclonal antibody (Product # PA5-14445) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). CDK4 immunoreactivity is localized to Cytoplasm significantly.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CDK4 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with CDK4 polyclonal antibody (Product # PA5-14445) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CDK4 in formalin-fixed and paraffin-embedded human prostate carcinoma. Samples were incubated with CDK4 polyclonal antibody (Product # PA5-14445) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HL-60 cells using a CDK4 polyclonal antibody (Product # PA5-14445) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CDK4 in HL60 cells (bottom histogram). Samples were incubated with CDK4 polyclonal antibody (Product # PA5-14445) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).