PA5-27827

PSK-J3

Antibody data

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Validation data

Product number: PA5-27827
Provider: Invitrogen Antibodies
Product name: Anti-CDK4 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-27827
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-27827 targets CDK4 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. The PA5-27827 immunogen is recombinant fragment corresponding to a region within amino acids 148 and 303 of Human CDK4.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.6 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK4 using 30 µg of 293T lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CDK4 polyclonal antibody (Product # PA5-27827) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK4 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CDK4 polyclonal antibody (Product # PA5-27827) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CDK4 was achieved by transfecting A-431 cells with CDK4 specific validated siRNA (Silencer® select Product # s2823). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK4 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-CDK4 Polyclonal Antibody (Product # PA5-27827, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK4.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), K-562 (Lane 3), HeLa (Lane 4) and A549 (Lane 5). The blot was probed with Anti-CDK4 Polyclonal Antibody (Product # PA5-27827, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A ~35 kDa band corresponding to CDK4 was observed across the cell lines tested. A non-specific band was also observed at around 55 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CDK4 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDK4 Polyclonal Antibody (Product # PA5-27827) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK4 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDK4 Polyclonal Antibody (Product # PA5-27827) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.