MA1-828

antibody from Invitrogen Antibodies

Targeting: VRK1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MA1-828

Provider

Invitrogen Antibodies

Product name

VRK1 Monoclonal Antibody (1F6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-828 detects VRK1 from human and mouse samples. MA1-828 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry applications. For Western blotting, it is recommended to block with 3% BSA (not milk). The MA1-828 immunogen is recombinant fusion protein GST-VRK1.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1F6

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of VRK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VRK1 (1F6) Mouse Monoclonal Antibody (Product # MA1-828) at 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VRK1 (green) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (left panel) or with (right panel) a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:200 overnight at 4°C, washed with PBS, and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VRK1 (green) in U251 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (left panel) or with (right panel) a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:200 overnight at 4C, washed with PBS, and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VRK1 (green) in murine cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (left panel) or with (right panel) a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:200 overnight at 4°C, washed with PBS, and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VRK1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.