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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
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- Product number
- HPA000660 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA000660, RRID:AB_1080579
- Product name
- Anti-VRK1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human VRK1, Gene description: vaccinia related kinase 1, Validated applications: WB, Uniprot ID: Q99986, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Analysis of Serial Neuroblastoma PDX Passages in Mice Allows the Identification of New Mediators of Neuroblastoma Aggressiveness
VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells
VRK1 Depletion Facilitates the Synthetic Lethality of Temozolomide and Olaparib in Glioblastoma Cells
Olaparib and ionizing radiation trigger a cooperative DNA-damage repair response that is impaired by depletion of the VRK1 chromatin kinase
VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis
Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation
VRK1 regulates Cajal body dynamics and protects coilin from proteasomal degradation in cell cycle
The Spinal Muscular Atrophy with Pontocerebellar Hypoplasia GeneVRK1Regulates Neuronal Migration through an Amyloid-β Precursor Protein-Dependent Mechanism
Vaccinia-related kinase 1 (VRK1) confers resistance to DNA-damaging agents in human breast cancer by affecting DNA damage response
Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes.
Rewiring of human lung cell lineage and mitotic networks in lung adenocarcinomas
Molecular genetic analysis of VRK1 in mammary epithelial cells: depletion slows proliferation in vitro and tumor growth and metastasis in vivo
Vaccinia-related Kinase 1 (VRK1) Is an Upstream Nucleosomal Kinase Required for the Assembly of 53BP1 Foci in Response to Ionizing Radiation-induced DNA Damage
Gómez-Muñoz M, Aguilar-Morante D, Colmenero-Repiso A, Amador-Álvarez A, Ojeda-Puertas M, Cordero Varela J, Rodríguez-Prieto I, Pardal R, Vega F
International Journal of Molecular Sciences 2023;24(2):1590
International Journal of Molecular Sciences 2023;24(2):1590
VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells
Menez V, Kergrohen T, Shasha T, Silva-Evangelista C, Le Dret L, Auffret L, Subecz C, Lancien M, Ajlil Y, Vilchis I, Beccaria K, Blauwblomme T, Oberlin E, Grill J, Castel D, Debily M
Frontiers in Oncology 2023;13
Frontiers in Oncology 2023;13
VRK1 Depletion Facilitates the Synthetic Lethality of Temozolomide and Olaparib in Glioblastoma Cells
Navarro-Carrasco E, Lazo P
Frontiers in Cell and Developmental Biology 2021;9
Frontiers in Cell and Developmental Biology 2021;9
Olaparib and ionizing radiation trigger a cooperative DNA-damage repair response that is impaired by depletion of the VRK1 chromatin kinase
Campillo-Marcos I, Lazo P
Journal of Experimental & Clinical Cancer Research 2019;38(1)
Journal of Experimental & Clinical Cancer Research 2019;38(1)
VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis
Moura D, Campillo-Marcos I, Vázquez-Cedeira M, Lazo P
Cellular and Molecular Life Sciences 2018;75(14):2591-2611
Cellular and Molecular Life Sciences 2018;75(14):2591-2611
Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation
Moura D, Fernández I, Marín-Royo G, López-Sánchez I, Martín-Doncel E, Vega F, Lazo P
Scientific Reports 2016;6(1)
Scientific Reports 2016;6(1)
VRK1 regulates Cajal body dynamics and protects coilin from proteasomal degradation in cell cycle
Cantarero L, Sanz-García M, Vinograd-Byk H, Renbaum P, Levy-Lahad E, Lazo P
Scientific Reports 2015;5(1)
Scientific Reports 2015;5(1)
The Spinal Muscular Atrophy with Pontocerebellar Hypoplasia GeneVRK1Regulates Neuronal Migration through an Amyloid-β Precursor Protein-Dependent Mechanism
Vinograd-Byk H, Sapir T, Cantarero L, Lazo P, Zeligson S, Lev D, Lerman-Sagie T, Renbaum P, Reiner O, Levy-Lahad E
The Journal of Neuroscience 2015;35(3):936-942
The Journal of Neuroscience 2015;35(3):936-942
Vaccinia-related kinase 1 (VRK1) confers resistance to DNA-damaging agents in human breast cancer by affecting DNA damage response
Salzano M, Vázquez-Cedeira M, Sanz-García M, Valbuena A, Blanco S, Fernández I, Lazo P
Oncotarget 2014;5(7):1770-1778
Oncotarget 2014;5(7):1770-1778
Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes.
Molitor TP, Traktman P
Molecular biology of the cell 2014 Mar;25(6):891-903
Molecular biology of the cell 2014 Mar;25(6):891-903
Rewiring of human lung cell lineage and mitotic networks in lung adenocarcinomas
Kim I, Quigley D, To M, Pham P, Lin K, Jo B, Jen K, Raz D, Kim J, Mao J, Jablons D, Balmain A
Nature Communications 2013;4(1)
Nature Communications 2013;4(1)
Molecular genetic analysis of VRK1 in mammary epithelial cells: depletion slows proliferation in vitro and tumor growth and metastasis in vivo
Molitor T, Traktman P
Oncogenesis 2013;2(6):e48-e48
Oncogenesis 2013;2(6):e48-e48
Vaccinia-related Kinase 1 (VRK1) Is an Upstream Nucleosomal Kinase Required for the Assembly of 53BP1 Foci in Response to Ionizing Radiation-induced DNA Damage
Sanz-García M, Monsalve D, Sevilla A, Lazo P
Journal of Biological Chemistry 2012;287(28):23757-23768
Journal of Biological Chemistry 2012;287(28):23757-23768
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-VRK1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
