Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 11-0058-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD5 Monoclonal Antibody (L17F12), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The L17F12 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation. Applications Reported: This L17F12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This L17F12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- L17F12
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Correlation of APRIL with production of inflammatory cytokines during acute malaria in the Brazilian Amazon.
High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.
Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.
Pinna RA, Dos Santos AC, Perce-da-Silva DS, da Silva LA, da Silva RNR, Alves MR, Santos F, de Oliveira Ferreira J, Lima-Junior JC, Villa-Verde DM, De Luca PM, Carvalho-Pinto CE, Banic DM
Immunity, inflammation and disease 2018 Jun;6(2):207-220
Immunity, inflammation and disease 2018 Jun;6(2):207-220
High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.
O'Connor D, Clutterbuck EA, Thompson AJ, Snape MD, Ramasamy MN, Kelly DF, Pollard AJ
Genome medicine 2017 Jan 30;9(1):11
Genome medicine 2017 Jan 30;9(1):11
Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.
Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R
Proceedings of the National Academy of Sciences of the United States of America 1981 Mar;78(3):1791-5
Proceedings of the National Academy of Sciences of the United States of America 1981 Mar;78(3):1791-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD5 FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye