Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-0059-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD5 Monoclonal Antibody (UCHT2), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation. The monoclonal antibody UCHT2 recognizes primate CD5. Applications Reported: The UCHT2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- UCHT2
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
A pathobiological role of the insulin receptor in chronic lymphocytic leukemia.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
Lim AI, Li Y, Lopez-Lastra S, Stadhouders R, Paul F, Casrouge A, Serafini N, Puel A, Bustamante J, Surace L, Masse-Ranson G, David E, Strick-Marchand H, Le Bourhis L, Cocchi R, Topazio D, Graziano P, Muscarella LA, Rogge L, Norel X, Sallenave JM, Allez M, Graf T, Hendriks RW, Casanova JL, Amit I, Yssel H, Di Santo JP
Cell 2017 Mar 9;168(6):1086-1100.e10
Cell 2017 Mar 9;168(6):1086-1100.e10
A pathobiological role of the insulin receptor in chronic lymphocytic leukemia.
Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, Erba H, Campagnaro E, Shedden K, Kaminski M, Malek SN
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 May 1;17(9):2679-92
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 May 1;17(9):2679-92
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD5 FITC (filled histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye