Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-0059-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD5 Monoclonal Antibody (UCHT2), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation. The monoclonal antibody UCHT2 recognizes primate CD5. Applications Reported: The UCHT2 antibody has been reported for use in flow cytometric analysis. Applications Tested: The UCHT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- UCHT2
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Heightened BTK-dependent cell proliferation in unmutated chronic lymphocytic leukemia confers increased sensitivity to ibrutinib.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
The 1F7 idiotype is selectively expressed on CD5+ B cells and elevated in chronic hepatitis C virus infection.
Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Heightened BTK-dependent cell proliferation in unmutated chronic lymphocytic leukemia confers increased sensitivity to ibrutinib.
Guo A, Lu P, Galanina N, Nabhan C, Smith SM, Coleman M, Wang YL
Oncotarget 2016 Jan 26;7(4):4598-610
Oncotarget 2016 Jan 26;7(4):4598-610
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The 1F7 idiotype is selectively expressed on CD5+ B cells and elevated in chronic hepatitis C virus infection.
Davtyan TK, Hovsepyan MP, Mkhitaryan LM, Hakobyan GS, Brazil A, Barrett L, Hirsch G, Peltekian KM, Grant MD
Immunology and cell biology 2009 Aug-Sep;87(6):457-63
Immunology and cell biology 2009 Aug-Sep;87(6):457-63
Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold.
Fan CY, Huang CC, Chiu WC, Lai CC, Liou GG, Li HC, Chou MY
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Nov;22(11):3795-804
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Nov;22(11):3795-804
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with APC Anti-Human CD19 (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD5 PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye