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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12-0059-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD5 Monoclonal Antibody (UCHT2), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.
- Conjugate
- Yellow dye
- Antibody clone number
- UCHT2
- Concentration
- 5 µL/Test
Submitted references Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Dual SYK/JAK inhibition overcomes ibrutinib resistance in chronic lymphocytic leukemia: Cerdulatinib, but not ibrutinib, induces apoptosis of tumor cells protected by the microenvironment.
Heightened BTK-dependent cell proliferation in unmutated chronic lymphocytic leukemia confers increased sensitivity to ibrutinib.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
The 1F7 idiotype is selectively expressed on CD5+ B cells and elevated in chronic hepatitis C virus infection.
Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Dual SYK/JAK inhibition overcomes ibrutinib resistance in chronic lymphocytic leukemia: Cerdulatinib, but not ibrutinib, induces apoptosis of tumor cells protected by the microenvironment.
Guo A, Lu P, Coffey G, Conley P, Pandey A, Wang YL
Oncotarget 2017 Feb 21;8(8):12953-12967
Oncotarget 2017 Feb 21;8(8):12953-12967
Heightened BTK-dependent cell proliferation in unmutated chronic lymphocytic leukemia confers increased sensitivity to ibrutinib.
Guo A, Lu P, Galanina N, Nabhan C, Smith SM, Coleman M, Wang YL
Oncotarget 2016 Jan 26;7(4):4598-610
Oncotarget 2016 Jan 26;7(4):4598-610
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The 1F7 idiotype is selectively expressed on CD5+ B cells and elevated in chronic hepatitis C virus infection.
Davtyan TK, Hovsepyan MP, Mkhitaryan LM, Hakobyan GS, Brazil A, Barrett L, Hirsch G, Peltekian KM, Grant MD
Immunology and cell biology 2009 Aug-Sep;87(6):457-63
Immunology and cell biology 2009 Aug-Sep;87(6):457-63
Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold.
Fan CY, Huang CC, Chiu WC, Lai CC, Liou GG, Li HC, Chou MY
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Nov;22(11):3795-804
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Nov;22(11):3795-804
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with APC Anti-Human CD19 (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD5 PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 HEBCan Rescues Hematopoiesis and T Cell Development in HEB -/- hESCs in OP9-DL4 Co-cultures (A) qRT-PCR analysis for the expression of hematopoietic genes in CD34 + cells sorted from WT, KO + GFP, and KO + HEBCan day-8 (d8) EBs. mRNA levels are shown relative to GAPDH. (B and C) Percentages (B) and numbers (C) of CD45 + cells in d12 and d18 OP9-DL4 co-cultures. (D and E) Flow-cytometric analysis of T cell development from WT, KO + GFP, and KO + HEBCan d8 EB-derived CD34 + cells at d12 (D) and d18 (E) of OP9-DL4 co-culture. Cells are gated on the CD45 + DAPI - population. Error bars represent mean +- SD (n = 3 independent experiments). * p < 0.05, ** p < 0.01, *** p < 0.005 by Student's t test. Plots in (B), (D), and (E) are representative of three independent experiments.
- Conjugate
- Yellow dye
