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- Antigen structure
- References [1]
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- Product number
- MA1-10608 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neutrophil elastase Monoclonal Antibody (ELA10-101.5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-10608 detects Neutrophil elastase from human samples. MA1-10608 has been successfully used in ELISA and in studies of inhibition and enzyme kinetics of neutrophil elastase. The MA1-10608 immunogen is purified human Neutrophil Elastase.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ELA10-101.5
- Vial size
- 500 µg
- Concentration
- 1.7 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa.
Floyd M, Winn M, Cullen C, Sil P, Chassaing B, Yoo DG, Gewirtz AT, Goldberg JB, McCarter LL, Rada B
PLoS pathogens 2016 Nov;12(11):e1005987
PLoS pathogens 2016 Nov;12(11):e1005987
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig 2 Flagellum is the main contributor to NET release triggered by P . aeruginosa . (A) Human PMNs were exposed to WT (PAK or PAO1) and flagellum-deficient strains (PAK flgC and PAO1 fliC ) of P . aeruginosa (10 MOI), and the following readouts were measured: (A) Release of myeloperoxidase (MPO) and human neutrophil elastase (HNE) measured by ELISA. MPO: PAK ( n = 5), PAO1 ( n = 4); HNE: PAK ( n = 11), PAO1 ( n = 7). Values without bacterial stimulation were subtracted. (B) Enzymatic activity of MPO: PAK ( n = 7), PAO1 ( n = 7). Values without bacterial stimulation were subtracted. (C) DNA release (Sytox Orange fluorescence): PAO1 ( n = 13), PAK ( n = 3). (D) NET release measured by ELISA. MPO-DNA: PAK ( n = 3), PAO1 ( n = 5); HNE-DNA: PAK ( n = 3), PAO1 ( n = 5). Mean+/-S.E.M. Values of unstimulated PMNs were subtracted. *, p
