MA1-10608

antibody from Invitrogen Antibodies

Targeting: ELANE ELA2, HLE, HNE, NE

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10608

Provider

Invitrogen Antibodies

Product name

Neutrophil elastase Monoclonal Antibody (ELA10-101.5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1-10608 detects Neutrophil elastase from human samples.

Antibody clone number

ELA10-101.5

Concentration

0.9 mg/mL

References

Submitted references

Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa.

Floyd M, Winn M, Cullen C, Sil P, Chassaing B, Yoo DG, Gewirtz AT, Goldberg JB, McCarter LL, Rada B
PLoS pathogens 2016 Nov;12(11):e1005987

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 2 Flagellum is the main contributor to NET release triggered by P . aeruginosa . (A) Human PMNs were exposed to WT (PAK or PAO1) and flagellum-deficient strains (PAK flgC and PAO1 fliC ) of P . aeruginosa (10 MOI), and the following readouts were measured: (A) Release of myeloperoxidase (MPO) and human neutrophil elastase (HNE) measured by ELISA. MPO: PAK ( n = 5), PAO1 ( n = 4); HNE: PAK ( n = 11), PAO1 ( n = 7). Values without bacterial stimulation were subtracted. (B) Enzymatic activity of MPO: PAK ( n = 7), PAO1 ( n = 7). Values without bacterial stimulation were subtracted. (C) DNA release (Sytox Orange fluorescence): PAO1 ( n = 13), PAK ( n = 3). (D) NET release measured by ELISA. MPO-DNA: PAK ( n = 3), PAO1 ( n = 5); HNE-DNA: PAK ( n = 3), PAO1 ( n = 5). Mean+/-S.E.M. Values of unstimulated PMNs were subtracted. *, p