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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- NB100-64392 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64392, RRID:AB_963322
- Product name
- Mouse Monoclonal Neutrophil Elastase/ELA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64392 is specific for human neutrophil elastase.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Monoclonal anti-neutrophil elastase antibody characterisation: ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granules.
Apoptosis-induced proteinase 3 membrane expression is independent from degranulation.
Davies PL, Maxwell NC, Kotecha S, Spiller OB
Journal of immunological methods 2008 Jul 31;336(2):175-82
Journal of immunological methods 2008 Jul 31;336(2):175-82
Apoptosis-induced proteinase 3 membrane expression is independent from degranulation.
Durant S, Pederzoli M, Lepelletier Y, Canteloup S, Nusbaum P, Lesavre P, Witko-Sarsat V
Journal of leukocyte biology 2004 Jan;75(1):87-98
Journal of leukocyte biology 2004 Jan;75(1):87-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.
