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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-29659 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neutrophil elastase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A Pilot Study to Investigate the Balance between Proteases and α1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients.
Cagnone M, Piloni D, Ferrarotti I, Di Venere M, Viglio S, Magni S, Bardoni A, Salvini R, Fumagalli M, Iadarola P, Martinello S, Meloni F
High-throughput 2019 Feb 13;8(1)
High-throughput 2019 Feb 13;8(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Neutrophil elastase Polyclonal Antibody (Product # PA5-29659). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: HepG2. 12% SDS PAGE. Neutrophil elastase Polyclonal Antibody (Product # PA5-29659) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Neutrophil elastase Polyclonal Antibody (Product # PA5-29659). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: HepG2. 12% SDS PAGE. Neutrophil elastase Polyclonal Antibody (Product # PA5-29659) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Neutrophil elastase Polyclonal Antibody (Product # PA5-29659). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Neutrophil elastase Polyclonal Antibody (Product # PA5-29659) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Neutrophil elastase was performed in HepG2 whole cell extracts using 5 µg of Neutrophil elastase Polyclonal Antibody (Product # PA5-29659). Samples were transferred to a membrane and probed with Neutrophil elastase Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
