100-401-862

antibody from Rockland Immunochemicals, Inc.

Targeting: PIK3CD p110D

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-862

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-862, RRID:AB_1961753

Product name

Anti-PI3-kinase p110 ? (delta subunit) (RABBIT) Antibody - 100-401-862

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunoprecipitation and western blot using ROCKLAND Immunochemical's Rabbit-anti-PI3K p110d antibody. Lane 1 shows the detection of a single band corresponding to mouse p110d detected in a lysate from p110d +/+ mice (lane 1) and the absence of staining in a similar lysate isolated from p110d -/- mice (lane 2). Molecular weight markers confirm a MW of ~120 kDa. In both instances 2 mg of a total splenic lysate was used for immunoprecipitation and western blot analysis. For IP use ~5 µl of antiserum. For WB use a 1:5,000 dilution of antiserum. Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) with visualization via ECL. Film exposure approximately 45''. Other detection systems will yield similar results. See Jou et al for additional details.

Validation comment

Western Blot