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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA501173 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501173, RRID:AB_11129814
- Product name
- Anti-CYP1A2 (Cytochrome P450 1A2) mouse monoclonal antibody, clone OTI7D12 (formerly 7D12)
- Antibody type
- Monoclonal
- Description
- Anti-CYP1A2 (Cytochrome P450 1A2) mouse monoclonal antibody, clone OTI7D12 (formerly 7D12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CYP1A2
- Isotype
- IgG
- Antibody clone number
- OTI7D12
- Vial size
- 100 µl
- Concentration
- 0.73 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (RC221636, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CYP1A2 mouse monoclonal antibody (TA501173) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2(RC221636).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CYP1A2 mouse monoclonal antibody (TA501173).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501173, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501173, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC221636 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP1A2 antibody(TA501173), and then analyzed by flow cytometry.
- Validation comment
- FC
