Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C19057 - Provider product page
- Provider
- LSBio
- Product name
- CIITA Antibody LS-C19057
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
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- Experimental details
- Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA. In panel A, both samples on PVDF were incubated with 10 mg/mL mouse anti-FLAG antibody (Sigma) for 45'. In panel B, both samples on PVDF were incubated with a 1:500 dilution of anti-CIITA (1-333) for 45'.