TA319304

antibody from OriGene

Targeting: CIITA C2TA, MHC2TA, NLRA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319304

Provider

OriGene

Product name

Rabbit polyclonal anti-CIITA antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-CIITA antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

CIITA

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

90mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB of anti-CIITA against Cos7 cells lysates after transfection with FLAG-CIITA-336-884 or HA-CIITA. A: both samples on PVDF were incubated with 10ug/ml mouse anti-FLAG followed by ALP rabbit anti-mouse IgG at 200ug/mL. Result shows the overexpression of FLAG-CIITA-336-842 in Cos7 cells. B: both samples on PVDF were incubated with the ab at 1:500 for 45'. Result shows anti-CIITA (1-333) is specific for aa 1-333 of CIITA and that the antibody is not cross reactive with the FLAG portion of the immunogen.

Validation comment

WB