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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA506234 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506234, RRID:AB_2623693
- Product name
- CD19 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)
- Antibody type
- Monoclonal
- Description
- CD19 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CD19
- Isotype
- IgG
- Antibody clone number
- OTI3G7
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum.
Bagashev A, Sotillo E, Tang CH, Black KL, Perazzelli J, Seeholzer SH, Argon Y, Barrett DM, Grupp SA, Hu CC, Thomas-Tikhonenko A
Molecular and cellular biology 2018 Nov 1;38(21)
Molecular and cellular biology 2018 Nov 1;38(21)
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CD19 mouse monoclonal antibody (TA506234) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD19(RC202922).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD19 mouse monoclonal antibody. (TA506234; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of living K562 cells,using anti-CD19 antibody(TA506234),(Red),compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
