Antibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-0199-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD19 Monoclonal Antibody (HIB19), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vitro functional studies. (Please use Functional Grade Purified HIB19, cat. 16-0199, in functional assays. Fluorochrome-conjugated HIB19 is recommended for use in flow cytometry.). Applications Tested: This HIB19 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HIB19
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.
A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.
Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function.
Chimeric antigen receptor-engineered cytokine-induced killer cells overcome treatment resistance of pre-B-cell acute lymphoblastic leukemia and enhance survival.
Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.
IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require α4β1 Integrin To Migrate between the Peritoneum and Inflamed Skin.
Medial HOXA genes demarcate haematopoietic stem cell fate during human development.
CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.
Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection.
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccination.
dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.
Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.
Thymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes.
Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36.
Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis.
The role of transforming growth factor β signaling in fibroblast-like synoviocytes from patients with oligoarticular juvenile idiopathic arthritis: dysregulation of transforming growth factor β signaling, including overexpression of bone morphogenetic protein 4, may lead to a chondrocyte phenotype and may contribute to bony hypertrophy.
Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.
Replication and meta-analysis of GWAS identified susceptibility loci in Kawasaki disease confirm the importance of B lymphoid tyrosine kinase (BLK) in disease susceptibility.
Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.
Cytomegalovirus-specific T-cell reactivity in biliary atresia at the time of diagnosis is associated with deficits in regulatory T cells.
A pathobiological role of the insulin receptor in chronic lymphocytic leukemia.
Generation of bivalent chromatin domains during cell fate decisions.
Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad.
Role played by the programmed death-1-programmed death ligand pathway during innate immunity against Mycobacterium tuberculosis.
Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.
Improved serum-free culture conditions for the differentiation of human and murine fibrocytes.
Lung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease.
Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.
Liu J, Huang X, Hao S, Wang Y, Liu M, Xu J, Zhang X, Yu T, Gan S, Dai D, Luo X, Lu Q, Mao C, Zhang Y, Shen N, Li B, Huang M, Zhu X, Jin J, Cheng X, Sun SC, Xiao Y
Nature communications 2018 Mar 19;9(1):1136
Nature communications 2018 Mar 19;9(1):1136
A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.
Böiers C, Richardson SE, Laycock E, Zriwil A, Turati VA, Brown J, Wray JP, Wang D, James C, Herrero J, Sitnicka E, Karlsson S, Smith AJH, Jacobsen SEW, Enver T
Developmental cell 2018 Feb 5;44(3):362-377.e7
Developmental cell 2018 Feb 5;44(3):362-377.e7
Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.
Magri G, Comerma L, Pybus M, Sintes J, Lligé D, Segura-Garzón D, Bascones S, Yeste A, Grasset EK, Gutzeit C, Uzzan M, Ramanujam M, van Zelm MC, Albero-González R, Vazquez I, Iglesias M, Serrano S, Márquez L, Mercade E, Mehandru S, Cerutti A
Immunity 2017 Jul 18;47(1):118-134.e8
Immunity 2017 Jul 18;47(1):118-134.e8
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
Zhu N, Wang H, Wang B, Wei J, Shan W, Feng J, Huang H
Stem cells international 2016;2016:5687589
Stem cells international 2016;2016:5687589
The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function.
van der Aa E, Buschow SI, Biesta PJ, Janssen HL, Woltman AM
PloS one 2016;11(8):e0161235
PloS one 2016;11(8):e0161235
Chimeric antigen receptor-engineered cytokine-induced killer cells overcome treatment resistance of pre-B-cell acute lymphoblastic leukemia and enhance survival.
Oelsner S, Wagner J, Friede ME, Pfirrmann V, Genßler S, Rettinger E, Buchholz CJ, Pfeifer H, Schubert R, Ottmann OG, Ullrich E, Bader P, Wels WS
International journal of cancer 2016 Oct 15;139(8):1799-809
International journal of cancer 2016 Oct 15;139(8):1799-809
Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.
Yiwen Z, Shilin G, Yingshi C, Lishi S, Baohong L, Chao L, Linghua L, Ting P, Hui Z
Oncotarget 2016 Nov 22;7(47):77732-77748
Oncotarget 2016 Nov 22;7(47):77732-77748
IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require α4β1 Integrin To Migrate between the Peritoneum and Inflamed Skin.
Geherin SA, Gómez D, Glabman RA, Ruthel G, Hamann A, Debes GF
Journal of immunology (Baltimore, Md. : 1950) 2016 Mar 15;196(6):2514-2525
Journal of immunology (Baltimore, Md. : 1950) 2016 Mar 15;196(6):2514-2525
Medial HOXA genes demarcate haematopoietic stem cell fate during human development.
Dou DR, Calvanese V, Sierra MI, Nguyen AT, Minasian A, Saarikoski P, Sasidharan R, Ramirez CM, Zack JA, Crooks GM, Galic Z, Mikkola HK
Nature cell biology 2016 Jun;18(6):595-606
Nature cell biology 2016 Jun;18(6):595-606
CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.
Jacoby E, Nguyen SM, Fountaine TJ, Welp K, Gryder B, Qin H, Yang Y, Chien CD, Seif AE, Lei H, Song YK, Khan J, Lee DW, Mackall CL, Gardner RA, Jensen MC, Shern JF, Fry TJ
Nature communications 2016 Jul 27;7:12320
Nature communications 2016 Jul 27;7:12320
Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection.
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, Raghunath A, Jeffers A, Tvinnereim AR, Schechter ME, Andrade BB, Mackman N, Idell S, Vankayalapati R
European journal of immunology 2016 Feb;46(2):464-79
European journal of immunology 2016 Feb;46(2):464-79
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
Yao Y, Deng Q, Song W, Zhang H, Li Y, Yang Y, Fan X, Liu M, Shang J, Sun C, Tang Y, Jin X, Liu H, Huang B, Zhou Y
Stem cell reports 2016 Aug 9;7(2):249-62
Stem cell reports 2016 Aug 9;7(2):249-62
A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccination.
Hoek KL, Samir P, Howard LM, Niu X, Prasad N, Galassie A, Liu Q, Allos TM, Floyd KA, Guo Y, Shyr Y, Levy SE, Joyce S, Edwards KM, Link AJ
PloS one 2015;10(2):e0118528
PloS one 2015;10(2):e0118528
dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.
Lim S, Kim WJ, Kim YH, Lee S, Koo JH, Lee JA, Yoon H, Kim DH, Park HJ, Kim HM, Lee HG, Yun Kim J, Lee JU, Hun Shin J, Kyun Kim L, Doh J, Kim H, Lee SK, Bothwell ALM, Suh M, Choi JM
Nature communications 2015 Sep 15;6:8244
Nature communications 2015 Sep 15;6:8244
Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.
Van Eyck L, De Somer L, Pombal D, Bornschein S, Frans G, Humblet-Baron S, Moens L, de Zegher F, Bossuyt X, Wouters C, Liston A
Arthritis & rheumatology (Hoboken, N.J.) 2015 Jun;67(6):1592-7
Arthritis & rheumatology (Hoboken, N.J.) 2015 Jun;67(6):1592-7
Thymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes.
Nunes-Cabaço H, Matoso P, Foxall RB, Tendeiro R, Pires AR, Carvalho T, Pinheiro AI, Soares RS, Sousa AE
Journal of virology 2015 Feb;89(4):2201-8
Journal of virology 2015 Feb;89(4):2201-8
Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36.
Krupna-Gaylord MA, Liveris D, Love AC, Wormser GP, Schwartz I, Petzke MM
PloS one 2014;9(6):e100174
PloS one 2014;9(6):e100174
Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis.
Todd SK, Pepper RJ, Draibe J, Tanna A, Pusey CD, Mauri C, Salama AD
Rheumatology (Oxford, England) 2014 Sep;53(9):1693-703
Rheumatology (Oxford, England) 2014 Sep;53(9):1693-703
The role of transforming growth factor β signaling in fibroblast-like synoviocytes from patients with oligoarticular juvenile idiopathic arthritis: dysregulation of transforming growth factor β signaling, including overexpression of bone morphogenetic protein 4, may lead to a chondrocyte phenotype and may contribute to bony hypertrophy.
Brescia AC, Simonds MM, McCahan SM, Fawcett PT, Rose CD
Arthritis & rheumatology (Hoboken, N.J.) 2014 May;66(5):1352-62
Arthritis & rheumatology (Hoboken, N.J.) 2014 May;66(5):1352-62
Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.
Preston SG, Majtán J, Kouremenou C, Rysnik O, Burger LF, Cabezas Cruz A, Chiong Guzman M, Nunn MA, Paesen GC, Nuttall PA, Austyn JM
PLoS pathogens 2013;9(6):e1003450
PLoS pathogens 2013;9(6):e1003450
Replication and meta-analysis of GWAS identified susceptibility loci in Kawasaki disease confirm the importance of B lymphoid tyrosine kinase (BLK) in disease susceptibility.
Chang CJ, Kuo HC, Chang JS, Lee JK, Tsai FJ, Khor CC, Chang LC, Chen SP, Ko TM, Liu YM, Chen YJ, Hong YM, Jang GY, Hibberd ML, Kuijpers T, Burgner D, Levin M, Burns JC, Davila S, International Kawasaki Disease Genetics Consortium., Korean Kawasaki Disease Genetics Consortium., Taiwan Kawasaki Disease Genetics Consortium., Chen YT, Chen CH, Wu JY, Lee YC
PloS one 2013;8(8):e72037
PloS one 2013;8(8):e72037
Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.
Barnig C, Cernadas M, Dutile S, Liu X, Perrella MA, Kazani S, Wechsler ME, Israel E, Levy BD
Science translational medicine 2013 Feb 27;5(174):174ra26
Science translational medicine 2013 Feb 27;5(174):174ra26
Cytomegalovirus-specific T-cell reactivity in biliary atresia at the time of diagnosis is associated with deficits in regulatory T cells.
Brindley SM, Lanham AM, Karrer FM, Tucker RM, Fontenot AP, Mack CL
Hepatology (Baltimore, Md.) 2012 Apr;55(4):1130-8
Hepatology (Baltimore, Md.) 2012 Apr;55(4):1130-8
A pathobiological role of the insulin receptor in chronic lymphocytic leukemia.
Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, Erba H, Campagnaro E, Shedden K, Kaminski M, Malek SN
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 May 1;17(9):2679-92
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 May 1;17(9):2679-92
Generation of bivalent chromatin domains during cell fate decisions.
De Gobbi M, Garrick D, Lynch M, Vernimmen D, Hughes JR, Goardon N, Luc S, Lower KM, Sloane-Stanley JA, Pina C, Soneji S, Renella R, Enver T, Taylor S, Jacobsen SE, Vyas P, Gibbons RJ, Higgs DR
Epigenetics & chromatin 2011 Jun 6;4(1):9
Epigenetics & chromatin 2011 Jun 6;4(1):9
Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad.
Frenzel A, Labi V, Chmelewskij W, Ploner C, Geley S, Fiegl H, Tzankov A, Villunger A
Blood 2010 Feb 4;115(5):995-1005
Blood 2010 Feb 4;115(5):995-1005
Role played by the programmed death-1-programmed death ligand pathway during innate immunity against Mycobacterium tuberculosis.
Alvarez IB, Pasquinelli V, Jurado JO, Abbate E, Musella RM, de la Barrera SS, García VE
The Journal of infectious diseases 2010 Aug 15;202(4):524-32
The Journal of infectious diseases 2010 Aug 15;202(4):524-32
Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
Pilling D, Fan T, Huang D, Kaul B, Gomer RH
PloS one 2009 Oct 16;4(10):e7475
PloS one 2009 Oct 16;4(10):e7475
B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.
Brudek T, Christensen T, Aagaard L, Petersen T, Hansen HJ, Møller-Larsen A
Retrovirology 2009 Nov 16;6:104
Retrovirology 2009 Nov 16;6:104
Improved serum-free culture conditions for the differentiation of human and murine fibrocytes.
Pilling D, Vakil V, Gomer RH
Journal of immunological methods 2009 Dec 31;351(1-2):62-70
Journal of immunological methods 2009 Dec 31;351(1-2):62-70
Lung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease.
Freeman CM, Martinez FJ, Han MK, Ames TM, Chensue SW, Todt JC, Arenberg DA, Meldrum CA, Getty C, McCloskey L, Curtis JL
American journal of respiratory and critical care medicine 2009 Dec 15;180(12):1179-88
American journal of respiratory and critical care medicine 2009 Dec 15;180(12):1179-88
Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.
Unsinger J, McDonough JS, Shultz LD, Ferguson TA, Hotchkiss RS
Journal of leukocyte biology 2009 Aug;86(2):219-27
Journal of leukocyte biology 2009 Aug;86(2):219-27
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (left) and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte gate were used for analysis.