Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [6]
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- Product number
- 17-0198-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD19 Monoclonal Antibody (SJ25C1), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SJ25C1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Survival, Retention, and Selective Proliferation of Lymphocytes Is Mediated by Gingival Fibroblasts.
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.
The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
Epithelial chemokine CXCL14 synergizes with CXCL12 via allosteric modulation of CXCR4.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Characterization of cell seeding and specific capture of B cells in microbubble well arrays.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Yan J, Pandey SP, Barnes BJ, Turner JR, Abraham C
Cell reports 2020 Jun 30;31(13):107820
Cell reports 2020 Jun 30;31(13):107820
Survival, Retention, and Selective Proliferation of Lymphocytes Is Mediated by Gingival Fibroblasts.
Moonen CGJ, Alders ST, Bontkes HJ, Schoenmaker T, Nicu EA, Loos BG, de Vries TJ
Frontiers in immunology 2018;9:1725
Frontiers in immunology 2018;9:1725
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.
Dai J, Luftig MA
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1727-1736
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1727-1736
The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
Albayati Z, Alyami A, Alomar S, Middleton D, Bonnett L, Aleem S, Flanagan BF, Christmas SE
Scandinavian journal of immunology 2017 Nov;86(5):396-407
Scandinavian journal of immunology 2017 Nov;86(5):396-407
Epithelial chemokine CXCL14 synergizes with CXCL12 via allosteric modulation of CXCR4.
Collins PJ, McCully ML, Martínez-Muñoz L, Santiago C, Wheeldon J, Caucheteux S, Thelen S, Cecchinato V, Laufer JM, Purvanov V, Monneau YR, Lortat-Jacob H, Legler DF, Uguccioni M, Thelen M, Piguet V, Mellado M, Moser B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Jul;31(7):3084-3097
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Jul;31(7):3084-3097
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Characterization of cell seeding and specific capture of B cells in microbubble well arrays.
Jones MC, Kobie JJ, Delouise LA
Biomedical microdevices 2013 Jun;15(3):453-63
Biomedical microdevices 2013 Jun;15(3):453-63
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD19 APC (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
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